| CatalogCode: | NB600-1101 |
| ProductName: | MeCP 2 Antibody |
| Product Description: | Rabbit Polyclonal anti-MeCP 2 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: M(1)VAGMLGLREEKSED(15)C, corresponding to amino acids 1-15 of Mouse MeCP2. |
| Specificity: | This antibody detects methyl CpG binding protein 2 (MeCP2). |
| CrossReactivity: | Cross-reacts with Mouse and Rat. Expected to cross-react with Human (100% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | This antibody can be used in Immunoprecipitaion, Immunofluoresence, and Western Blot. By Western Blot a 56kDa band is seen in AtT20 cells (predicted MW 52.4)Recommended Starting Dilutions*:WB: 0.5 ug/mlIF: 1:100 *Optimal dilutions should be determined by the end user. |
| Localization: | Nuclear |
| Background: | DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X-chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the Sin3 and histone deactylase protein families. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS with 1 mg/ml BSA containing 0.05% sodium azide |
| ListPrice: | 295 |
| AppSummary: | IF, IP, WB |
| SpeciesSummary: | Mu, Rt |
| ALTnames: | anti-AUTSX3 antibody, anti-DKFZp686A24160 antibody, anti-MECP 2 antibody, anti-MeCP 2 protein antibody, anti-Methyl CpG binding protein 2 (Rett syndrome) antibody, anti-Methyl CpG binding protein 2 antibody, anti-MRX16 antibody, anti-MRX79 antibody, anti-PPMX antibody, anti-RTS antibody, anti-RTT antibody |
| ProteinTarget: | MeCP 2 |
| PackageSize: | 0.1 mg |
| GeneralRef: | Ballestar E & Wolffe AP Methyl-CpG-binding proteins. Targeting specific gene repression. Eur J Biochem 268:1-6 (2001). Jones PL et al. Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription. Nat Genet 19:187-91 (1998). |