| Catalog Code: | NB600-1079 |
| Product Type: | Primary Antibody |
| Product Name: | BCRP Antibody |
| List Price: | 325 |
| Clonality: | Monoclonal |
| Gene ID: | 9429 |
| Host: | Rat |
| Clone: | BXP-9 |
| Isotype: | IgG1 |
| Product Description: | Rat Monoclonal anti-BCRP (BXP-9) |
| Cross Reactivity: | This antibody reacts with mouse. It does not react with human. Not yet tested in other species. |
| Species Summary: | Mu |
| Background: | An ABC transporter. Allows efflux of Hoechst dye, a property that has been used to separate bone marrow side population cells, which express BCRP/ABCG2. Appears to play a major role in the multidrug resistance phenotype of a specific MCF-7 breast cancer c |
| Alternate Names: | anti-ABCG2 antibody, anti-Breast Cancer Resistance Protein antibody |
| Immunogen: | Synthetic peptide, corresponding to amino acids 221-394 of mouse BCRP/ABCG2 fused to maltose binding protein. |
| Localization: | Plasma membrane, integral membrane protein |
| Specificity: | The BXP-9 Mab was selected after immunization with a fusion protein containing the E. coli maltose binding protein and a fragment of the mouse bcrp protein corresponding to amino acids 221-394. BXP-9 reacts with an internal epitope of bcrp, a 70 kD transm |
| Purity: | Tissue culture supernatant |
| Buffer: | 0.7% BSA, tissue culture supernatant (serum free), 0.1% Sodium Azide |
| Packaging: | 0.5 ml Tissue culture supernatant Rat ascites. |
| Package Size: | 0.5 ml |
| Concentration: | 0.25 mg/ml |
| Uses: | Immunocytochemistry: working dilution of 1:20 - 1:50 on acetone fixed cytospin preparations. Immunohistochemistry: BXP-9 (dilution of 1:20) on acetone fixed frozen sections can be followed by incubation with biotin-labeled rabbit anti-rat IgG (1:100, DAKO |
| Application Summary: | FACS, IHC, WB, ICC |