| request information: |
|
| Catalog Code: | NB600-1061 |
| Product Name: | CD10 Antibody |
| Product Description: | Mouse Monoclonal anti-CD10 (56C6) |
| Clone: | 56C6 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Prokaryotic recombinant fusion protein corresponding to the external of the CD10 glycoprotein |
| Cross Reactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.25 ml Tissue culture supernatant Mouse ascites. |
| Uses: | IHC:For an ABC system, incubate for 30-60 minutes at RT. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval before commencing with IHC. With high pH retrieval solutions, some tissues may require an avidin-biotin block. Overstaining with hematoxylin can mask staining, thus reducing overall contrast and making interpretation difficult. It is therefore recommended that a light hematoxylin stain be applied. Recently, CD10 has been used in a panel for mantle cell lymphoma with cyclin D1(+), CD43 (+), CD5 (+), IgM (+), CD23 (-) and CD10 (-). |
| Localization: | Cell membrane |
| Background: | Human CD10, also known as common acute lymphoblastic leukemia (CALLA), has been shown to react with TdT+ lymphoblastic leukemia, follicular germinal cell lymphoma, Burkitt?s lymphoma and chronic myelocytic leukemia. CD10 also marks normal early lymphoid progenitor cells, immature B-cells in adult bone marrow and germinal cells in normal tonsil and normal lymphoid tissue. It is also expressed in some non-lymphoid tissues such as fibroblasts, breast myoepithelium and brush border of kidney. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Tissue culture supernatant |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | PBS. pH 7.3 |
| Application Summary: | Immunohistochemistry-Paraffin 1:25-50 |
| Species Summary: | Hu, |
| Alternate Names: | anti-CALLA antibody, anti-CD 10 antibody, anti-Common acute lymphocytic leukemia antigen antibody, anti-Enkephalinase antibody, anti-gp100 antibody, anti-Membrane metalloendopeptidase antibody, anti-MME antibody, anti-Neprilysin antibody, anti-Neutral endopeptidase antibody, anti-Pmel17 antibody |
| Package Size: | 0.25 ml |
| Preservative: | Proprietary Preservative |
| Notes Main: | This antibody works well when used in combination with ab972 - Antigen retrieval solution, heat mediated high pH 9.5. |
| General References: | This product has been used in:Kaufmann O et al. Immunohistochemical detection of CD10 with monoclonal antibody 56C6 on paraffin sections. Am J Clin Pathol 111:117-22 (1999). Kurtin PJ et al. Demonstration of distinct antigenic profiles of small B-cell lymphomas by paraffin section immunohistochemistry. Am J Clin Pathol 112:319-29 (1999). General / background references:de Leon ED et al. Usefulness of an immunohistochemical panel in paraffin-embedded tissues for the differentiation of B-cell non-Hodgkin's lymphomas of small lymphocytes. Mod Pathol 11:1046-51 (1998). de Boer CJ et al. Bcl-1/cyclin D1 in malignant lymphoma. Ann Oncol 8 Suppl 2:109-17 (1997). |
order or
more information: | company product webpage |