| CatalogCode: | NB600-101 |
| ProductName: | Calreticulin Antibody |
| Product Description: | Rabbit Polyclonal anti-Calreticulin |
| Clonality: | Polyclonal |
| Immunogen: | A fusion protein to mouse calreticulin. |
| Specificity: | This antibody is specific for calreticulin. |
| CrossReactivity: | Human, bovine, rat, mouse and hamster calreticulin. Others not tested. |
| Packaging: | 0.1 ml Whole antisera Rabbit antisera. |
| Uses: | By Western Blot a band of ~ 55 kDa is seen. |
| Localization: | Calreticulin staining is localized to the ER in most cell types and under most conditions. |
| Background: | Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER). It is also a molecular chaperone and is involved in protein folding events in the ER. Calreticulin has also been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells. (2-3) Recently it has been shown that calreticulin is induced in response to various types of cell stress including amino acid deprivation. (1) Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and crucially participate in the coordination of these functions by the cell.(2-3) |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | n/a |
| Host_Name: | Rabbit |
| Buffer: | Phosphate buffered saline |
| ListPrice: | 285 |
| NovusRef: | 1. Placantonakis, D., et al. A dominant mutation of neuronal connexin 36 that blocks intercellular permeability. Molecular Brain Research. 98:15-28, 2002. 2. Luo, X., et al. C1q-calreticulin induced oxidation neurotoxicity: relevance for the neuropathoganesis of Alzheimer's disease. Journal of Neuroimmunology. 135:62-71, 2003. 3. Somogyi, E., et al. Calreticulin-an endoplasmic reticulum protein with calcium-binding activity is also found in the extracellular matrix. Matrix Biology. 22:179-191, 2003. 4. Woods, A., et al. Paxillin Associates with Poly (A)-binding Protein 1 at the Dense Endoplasmic Reticulum and the Leading Edge of Migrating Cells. Journal of Biological Chemistry. 277(8):6428-6437, 2002. 5. Ho, H, Suarez, S: Characterization of the Intracellular Calcium Store at the Base of the Sperm Flagellum That Regulates Hyperactivated Motility. Biology of Reproduction 68, 1590-1596 (2003) 6. Khanna, R., et al. Transient calnexin interaction confers long-term stability on folded K+ channel protein in the ER. Journal of Cell Science. 117: 2897-2908, 2004. 7. Knox, W., et al. Novel role of phospholipase C-delta1: regulation of liver mitochondrial Ca(2+) uptake. Am J. Physiol Gastrointest. Liver Physiol. 287: 533-540, 2004. 8. Olesen, SH., et al. Human FK506 binding protein 65 is associated with colorectal cancer. Mol. & Cell. Proteomics. 4.4: 534-544, 2005. (Immunohistochemistry, immunofluorescence, western blot, human) 9. Tsuzaka, K., et al. A splice variant of the TCR mRNA lacking exon 7 leads to the down-regulation of TCR, the TCR/CD3 complex, and IL-2 production in systemic lupus erythmatosus T cells. J. Immunol. 174:3518-3525, 2005. 10. Zhou, M., et al. ATP-sensitive K+-channel subunits on the mitochondeia and endoplasmic reticulum of rat cardiomyocytes. J. Histochem. Cytochem. 53:1491-1500, 2005. 11. Nurden, P., Debili, N., Vainchenker, W., Bobe, R., Bredoux, R., Corvazier, E., Combrie, R., Fressinaud, E., Meyer, D., Nurden, A. T., and Enouf, J. (2006) Impaired megakaryocytopoiesis in type 2B von Willebrand disease with severe thrombocytopenia, 108, 2587-2595. 12. Jain, P., et al. Identification of HTLV-1 tax amino acid signals and cellular factors involved in secretion of the viral oncoprotein. J Biol Chem. Sep 2007; 10.1074/jbc.M707317200. 13. Jain, P., et al. Identification of Human T Cell Leukemia Virus Type 1 Tax Amino Acid Signals and Cellular Factors Involved in Secretion of the Viral Oncoprotein. J. Biol. Chem, Nov 2007; 282:34581-34593. |
| AppSummary: | IHC, IF, IP, WB, ICC |
| SpeciesSummary: | Bv, Ha, Hu, Mu, Rt |
| ALTnames: | anti-Autoantigen RO antibody; anti-CALR antibody |
| ProteinTarget: | Calreticulin |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. R. Heal and J. McGivan. Induction of calreticulin expression in response to amino acid deprivation in Chinese hamster ovary cells. Biochemical Journal, 329: 389-394, 1998. 2. K-H Krause and M. Michalak. Calreticulin. Cell. 88: 439-443, 1997. 3. P.D. Nash et al, Calreticulin: not just another calcium-binding protein. Molecular and Cellular Biochemistry, 135: 71-78, 1994. 4. Llewellyn, D.H., et al. Calreticulin comes of age. trends in Cell Biology. 10: 399-402, 2000. |
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