| CatalogCode: | NB500-703 |
| ProductName: | Ku 80 Antibody |
| Product Description: | Mouse Monoclonal anti-Ku 80 (S10B1) |
| Clone: | S10B1 |
| Clonality: | Monoclonal |
| Immunogen: | Human placental extract. |
| Specificity: | This antibody reacts with a 80 kDa protein known as Ku (p80). |
| CrossReactivity: | Cross-reacts with Human and Monkey.Does not cross-react with Rabbit, Mouse or Cow.Not yet tested in other species. |
| Packaging: | 0.5 ml IgG purified Mouse ascites. |
| Uses: | IHC: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
| Localization: | Nuclear |
| Control: | Tonsil |
| Background: | Originally identified as an autoantigen, the Ku proteins are nuclear proteins found in eukaryotes. They exist as a heterodimer consisting of Ku 70 and Ku 80, and migrate at approximately 70 and 80 kDa, respectively. They bind to DNA ends at double strand breaks. The Ku proteins interact with the DNA dependent protein kinase (DNA PK), and participate in the targeting of DNA PK to DNA. The Ku proteins may participate in numerous cellular processes, including DNA repair and replication, cell signaling, and proliferation, transcriptional activation, and apoptosis |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | IgG purified |
| Isotype: | IgG1 kappa |
| Host_Name: | Mouse |
| Buffer: | PBS |
| ListPrice: | 295 |
| AppSummary: | IHC |
| SpeciesSummary: | Bv(-), Hu, Mu(-), Mk, Rb(-) |
| ALTnames: | anti-ATP dependent DNA helicase II 80 kDa subunit antibody, anti-ATP dependent DNA helicase II 86 Kd subunit antibody, anti-CTC box binding factor 85 kDa antibody, anti-CTC85 antibody, anti-CTCBF antibody, anti-DNA repair protein XRCC5 antibody, anti-KARP1 antibody, anti-Ku86 autoantigen related protein 1 antibody, anti-NFIV antibody, anti-Nuclear factor IV antibody, anti-Thyroid lupus autoantigen antibody, anti-TLAA antibody, anti-Xray repair complementing defective repair in Chinese hamster cells 5 antibody |
| ProteinTarget: | Ku80 |
| PackageSize: | 0.5 ml |
| GeneralRef: | This product has been used in:Ajmani AK et al. Absence of autoantigen Ku in mature human neutrophils and human promyelocytic leukemia line (HL-60) cells and lymphocytes undergoing apoptosis. J Exp Med 181:2049-58 (1995). Wang J et al. Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens. Mol Biol Rep 18:15-28 (1993). Knuth MW et al. Purification and characterization of proximal sequence element-binding protein 1, a transcription activating protein related to Ku and TREF that binds the proximal sequence element of the human U1 promoter. J Biol Chem 265:17911-20 (1990). |
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