| CatalogCode: | NB500-641 |
| ProductName: | Cytokeratin 18 Antibody |
| Product Description: | Mouse Monoclonal anti-Cytokeratin 18 (DC-10), conjugated to Biotin |
| Clone: | DC-10 |
| Clonality: | Monoclonal |
| Immunogen: | Tissue / cell preparation (Human) - human breast carcinoma cell line PMC-42 |
| Specificity: | NB 500-641 recognises cytokeratin peptide 18 (45 kDa) in human tissue. |
| CrossReactivity: | Reacts with Human. Not yet tested in other species. |
| Packaging: | 0.1 mg IgG purified Mouse ascites. |
| Uses: | This antibody can be used for Western blot, Immunoprecipitation, ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin-embedded sections). Not yet tested in other applications. Recommended starting dilutions:*ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IHC-P: 1/10 - 1/100. IP: Use at an assay dependent dilution. WB: 1/500. Predicted molecular weight: 47 kDa. *Optimal dilutions/concentrations should be determined by the end user. |
| Background: | Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (ie. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Constituents: PBS. pH 7.4 |
| ListPrice: | 285 |
| AppSummary: | ELISA, IHC-P, IP, WB, ICC |
| SpeciesSummary: | Hu |
| ALTnames: | anti-CK 18 antibody, anti-CYK18 antibody, anti-Cytokeratin endo B antibody, anti-Keratin 18 antibody, anti-Keratin D antibody, anti-Keratin type I cytoskeletal 18 antibody, anti-Kerd antibody, anti-KRT18 antibody |
| ProteinTarget: | Cytokeratin 18 |
| PackageSize: | 0.1 mg |
| Conjugate: | Biotin |
| GeneralRef: | Lauerov |