| CatalogCode: | NB500-592 |
| ProductName: | CD38 Antibody |
| Product Description: | Mouse Monoclonal anti-CD38 (HIT2), conjugated FITC |
| Clone: | HIT2 |
| Clonality: | Monoclonal |
| Immunogen: | Human foetal thymocytes |
| CrossReactivity: | Reacts with Human. Not yet tested in other species. |
| Packaging: | 2 ml of IgG1 purified antisera |
| Uses: | This antibody can be used for FACS. Not tested in other applications.Recommended starting dilutions:*FACS: Use a maximum of 5ul to label 106 cells. Analyse cells within 18 hours of staining. Use diluted reagent within 1 week.*Optimal dilutions/concentrations should be determined by the end user. |
| Background: | CD38 (T10) is a single chain 42 to 46 kDa type II integral transmembrane glycoprotein with a short N terminal cytoplasmic tail. CD38 is highly expressed on thymocytes. It is also expressed by early cells of B and T lineages, NK cells, plasma cells, monocytes and macrophages and may be detected on cells from multiple myeloma, ALL (B and T) and some AML. It is expressed by murine, but not human, hematopoietic progenitors. In normal lymph nodes and tonsils the antigen is detected mainly on B cells in germinal centers and in plasma cells. The extracellular domain of the molecule shares a high homology sequence with Aplysia ADP ribosyl cyclase. CD38 functions as a multicatalytic ectoenzyme serving as ADP ribosyl cyclase, ADPR hydrolase and possibly NAD+ glycohydrolase, or as a surface receptor. In mice, CD38 is expressed by follicular B cells, is down regulated on germinal centre B cells, and is not expressed by mature plasma cells. Murine CD38 is also expressed by all Mac1+ macrophages in the peritoneal cavities of unimmunized mice, but not by unstimulated bone marrow derived macrophages. This pattern of expression contrasts strikingly with that seen in the human. Monoclonal antibodies to CD38 have been shown to be useful in subtyping of lymphomas and leukemias, inhibition of B lymphopoiesis, detection of plasma cells, protection of B cells from apoptosis, and as a marker for activated B and T cell proliferation. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Protein G purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Preservative: Sodium Azide, Constituents: PBS, 4 - 5mg/ml BSA |
| ListPrice: | 285 |
| AppSummary: | FACS |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Acute lymphoblastic leukemia cells antigen CD38 antibody, anti-ADP ribosyl cyclase antibody, anti-CADPr hydrolase 1 antibody, anti-CD38 antigen antibody, anti-Cyclic ADP ribose hydrolase antibody, anti-Ecto nicotinamide adenine dinucleotide glycohydrolase antibody, anti-I19 (mouse) antibody, anti-Lymphocyte differentiation antigen CD38 antibody, anti-NIMR5 antigen (mouse) antibody, anti-p45 antibody, anti-T10 antibody |
| ProteinTarget: | CD38 |
| PackageSize: | 2 ml (100 tests) |
| Conjugate: | FITC |
| GeneralRef: | Tedder TF et al. Discontinuous expression of a membrane antigen (HB-7) during B lymphocyte differentiation. Tissue Antigens 24:140-9 (1984). PubMed: 6440308 Terhorst C et al. Biochemical studies of the human thymocyte cell-surface antigens T6, T9 and T10. Cell 23:771-80 (1981). PubMed: 7013988 Bhan AK et al. Stages of B cell differentiation in human lymphoid tissue. J Exp Med 154:737-49 (1981). PubMed: 7024458 Reinherz EL et al. Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci U S A 77:1588-92 (1980). PubMed: 6966400 |
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