| CatalogCode: | NB500-57412 |
| ProductName: | Histone H3 Antibody |
| Product Description: | Mouse Monoclonal anti-Histone H3 (H3-P) [Ser10] |
| Clone: | H3-P |
| Clonality: | Monoclonal |
| Immunogen: | Synthetic peptide: ARTKQTARKSpTGGKAPRK conjugated to KLH, corresponding to amino acids 1-18 of Human Histone H3. |
| Specificity: | The antibody reacts with human histone H3 (See Juan et al. 1998 and 1999), phosphorylated near its N-terminus (at Ser10) and does not detect the non-phosphorylated epitope (See Juan et al.). |
| CrossReactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.1 mg IgG purified Mouse ascites. |
| Uses: | ICC: Use at an assay dependant concentration. (See Juan <i> et al.</i> 1998 and 1999). IF: Use at an assay dependant dilution. (See Sauve <i>et al.</i>). I-ELISA: Use at an assay dependant dilution. (See Juan <i> et al.</i> 1998 and Sauve <i>et al.</i>)WB: Use at an assay dependant concentration. (See Sauve <i>et al.</i>) |
| Localization: | Nuclear |
| Background: | This antibody is a useful tool to study molecular mechanisms associated with the G2 to M transition and chromatin condensation and for the screening of in vivo inhibitors of kinase(s) or phosphatase(s) involved in H3 phosphorylation or dephosphorylation.Mitogenic stimulation, oncogen transformation, orinduction of oncogenic ras expression is accompanied with increases in Ser-10 phosphorylation of the N-terminal domain H3 (ref 5).Indeed, it has been shown that phosphorylated H3 is associated with c-fos and c-myc genes in stimulated cells (ref 6).H3 phosphorylation may contribute to proto-oncogen induction by modulating chromatin structure and releasing blocks in elongation.Site-specific phosphorylation of histone H3 at serine 10 (Ser10) appears to occur exclusively during mitosis in mammalian cells. H3 dephosphorylation occurs quite rapidly after mitosis and Ser10 remains unphosphorylated throughout the remainder of interphase (See Ajiro et al. and Wolffe). |
| ProductRef: | 1.Juan, G., et al, Cytometry, 32; 71-77 (1998) 2.Juan, G., et al, Exp. Cell Res., 246; 212-220 (1999) 3. Sauve, et al. JCB. 145: 225-235, 1999. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG2a |
| Host_Name: | Mouse |
| Buffer: | 0.01M Na phosphate, 0.25M NaCl, pH7.6, 15mg/ml BSA |
| ListPrice: | 330 |
| AppSummary: | FACS, ELISA, IF, WB, DB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-H3 antibody, anti-HisH3 antibody, anti-His1H3 antibody, anti-H3/a antibody, anti-H3/b antibody, anti-H3 antibody, c antibody, anti-H3/d antibody, anti-H3/f antibody, anti-H3/h antibody, anti-H3/i antibody, anti-H3/j antibody, anti-H3/k antibody, anti-H3/l antibody, anti-Histone H3 antibody |
| PackageSize: | 0.1 mg |
| GeneralRef: | This product has been used in:Juan G et al. Histone H3 phosphorylation in human monocytes and during HL-60 cell differentiation. Exp Cell Res 246:212-20 (1999). Sauve DM et al. Phosphorylation-induced rearrangement of the histone H3 NH2-terminal domain during mitotic chromosome condensation. J Cell Biol 145:225-35 (1999). Juan G et al. Histone H3 phosphorylation and expression of cyclins A and B1 measured in individual cells during their progression through G2 and mitosis. Cytometry 32:71-7 (1998). General / background references:Wei Y et al. Phosphorylation of histone H3 is required for proper chromosome condensation and segregation. Cell 97:99-109 (1999). Chadee DN et al. Increased Ser-10 phosphorylation of histone H3 in mitogen-stimulated and oncogene-transformed mouse fibroblasts. J Biol Chem 274:24914-20 (1999). Wolffe, A Chromatin. Structure and function. Academic Press : (1992).Shibata K et al. Mitosis-specific histone H3 phosphorylation in vitro in nucleosome structures. Eur J Biochem 192:87-93 (1990). Ajiro K & Nishimoto T Specific site of histone H3 phosphorylation related to the maintenance of premature chromosome condensation. Evidence for catalytically induced interchange of the subunits. J Biol Chem 260:15379-81 (1985). Gurley LR et al. Sequential phsophorylation of histone subfractions in the Chinese hamster cell cycle. J Biol Chem 250:3936-44 (1975). |
| Phosphorylated: | Ser10 |
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