| CatalogCode: | | NB500-464 |
| ProductName: | | CD222 Antibody |
| Product Description: | | Mouse Monoclonal anti-CD222 (MEM-238) |
| Clone: | | MEM-238 |
| Clonality: | | Monoclonal |
| Immunogen: | | Vaccinia-M6PR/iGFIIR |
| Epitope: | | Recognizes an epitope between domains 2 and 5. |
| Specificity: | | CD222 antigen (human) |
| CrossReactivity: | | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | | 0.1 mg Affinity purified Mouse ascites. |
| Uses: | | FACS: Use at an assay dependant concentration. WB: Use at an assay dependant dilution. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Background: | | CD222 is a 250kDa transmembrane protein with a short cytoplasmic tail containing an internalization signal. CD222 was originally identified as a receptor for IGFII and M6P-containing proteins (e.g. lysosomal hydrolases). Lysosomal enzymes are sorted to lysosomes via CD222 either from the Golgi, where the enzymes acquire M6P, or from the extracellular space. The majority of CD222 molecules (approximately 90-95%) are located intracellularly, only 5-10% is present on the cell membrane. The internalization rate seems to be enhanced by ligand induced dimerization of CD222 as well as by phosphorylation of its cytoplasmic serine. CD222 is also a receptor for TGFbeta latency associated peptide (LAP), proliferin and may bind several molecules independently of M6P, including plasminogen, CD87 or retinoic acid. It is involved in activation of latent TGFbeta [PROW]. |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | | Affinity purified |
| Isotype: | | IgG1 |
| Host_Name: | | Mouse |
| Buffer: | | Preservative: 15mM Sodium AzideConstituents: PBS. pH 7.4 |
| ListPrice: | | 285 |
| NovusRef: | | 1. El-Shewy, H. M., M.-H. Lee, et al. (2007). The Insulin-like Growth Factor Type 1 and Insulin-like Growth Factor Type 2/Mannose-6-phosphate Receptors Independently Regulate ERK1/2 Activity in HEK293 Cells. 282: 26150-26157. |
| AppSummary: | | FACS, WB |
| SpeciesSummary: | | Hu |
| ProteinTarget: | | CD222 |
| PackageSize: | | 0.1 mg |
| GeneralRef: | | This product has been used in:Leksa V et al. The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem 277:40575-82 (2002). PUBMED:12189157 General/background references:Mason D. et al. Leukocyte typing VII <i>Oxford University Press: (2002). |
| more information: | | company product webpage |