| CatalogCode: | NB500-347 |
| ProductName: | NCAM Antibody |
| Product Description: | Mouse Monoclonal anti-NCAM (MEM-188), conjugated PE |
| Clone: | MEM-188 |
| Clonality: | Monoclonal |
| Immunogen: | Tissue / cell preparation (KG-1 human acute myelogenous leukaemia cell line). |
| Specificity: | Human NCAM. Reacts with the 180 kDa isoform characteristic for leukocytes. Reactivity with other NCAM isoforms has not been tested. |
| CrossReactivity: | Cross-reacts with Human, Mouse and Monkey.Not yet tested in other species. |
| Packaging: | 100 tests |
| Uses: | FACS: Use at an assay dependent dilution. Designed for analysis of human blood cells using 20ul reagent per 100ul whole blood. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Type I membrane protein |
| Background: | NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma. |
| Storage: | Store at 4C. Do not freeze. |
| Isotype: | IgG2a |
| Host_Name: | Mouse |
| Buffer: | 15mM Sodium Azide in PBS with 0.2% BSA. pH 7.4 |
| ListPrice: | 285 |
| AppSummary: | FACS |
| SpeciesSummary: | Hu, Mu, Mk |
| ALTnames: | anti-CD56 antibody, anti-Cell Adhesion Molecule Neural 1 antibody, anti-MSK39 antibody, anti-N CAM 120 antibody, anti-N CAM 140 antibody, anti-N CAM 180 antibody, anti-NCAM 1 antibody, anti-NCAM1 antibody, anti-Neural cell adhesion molecule 1 antibody |
| ProteinTarget: | NCAM Growth Cone |
| PackageSize: | 100 tests |
| Conjugate: | PE |
| GeneralRef: | Kishimoto T. et al. (Eds.) Leukocyte Typing VI., Garland Publishing Inc. (1997). |
| NotesMain: | Avoid prolonged exposure to the light. |
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