| CatalogCode: | NB500-253 |
| ProductName: | NOD2 Antibody |
| Product Description: | Rabbit Polyclonal anti-NOD2 |
| Clonality: | Polyclonal |
| Immunogen: | A synthetic peptide made to the C-terminal region of the human NOD2 protein sequence (between residues 1,000-1,040). |
| Specificity: | This antibody is specific for NOD2 protein. |
| CrossReactivity: | This antibody reacts with human protein. Other species have not been tested. The immunogen used for this antibody production has 100% homology with human and monkey protein. |
| Packaging: | 0.1 ml Affinity purified Rabbit antisera. |
| Uses: | This antibody has been tested for Western blot of NOD2 transfected 293T lysates where a band is seen at ~110 kDa. We did not see positive results probing endogenous protein using HT29 lysates. |
| Background: | NOD2 is a member of the apoptosis regulating protein family that includes caspase recruitment-domains, as well as Apaf-1 and NOD1. It contains two N-terminal CARDs, a nucleotide binding domain (NBD), and multiple C-terminal leucine-rich repeats (LRRs). NOD2 is expressed in monocytes (whereas NOD1 is expressed in multiple tissues). NOD2 plays a role in regulating NF-kappaB, acts as an intracellular receptor for bacterial lipopolysaccharides, and contributes to inflammatory bowel disease (IBD) and Crohn's disease. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Affinity purified |
| Host_Name: | Rabbit |
| Buffer: | Tris-glycine buffer containing 150mM NaCl. |
| ListPrice: | 285 |
| NovusRef: | Till, A., P. Rosenstiel, et al. (2008). A role for membrane-bound CD147 in NOD2-mediated recognition of bacterial cytoinvasion. J Cell Sci. 121: 487-495. |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-NOD-2 antibody, anti-NOD 2 antibody, anti-CARD15 antibody, anti-Caspase recruitment domain protein 15 antibody, anti-IBD1 antibody, anti-Inflammatory Bowel disease protein 1 antibody |
| ProteinTarget: | NOD2 |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Hisamatsu, T., et al. CARD15/NOD2 funstions as an antibacterial factor in human intestinal epithelial cells. Gastroenterology. 124(4):1145-1149, 2003. (Immunoprecipitation, Western Blot, Human) 2. Abbott. D.W., et al. New signaling step for key player in chron's disease. Cell Press. 2004. 3. Abbott, D.W., et al. The Crohn's disease protein, NOD2, requires RIP2 in order to induce ubiquitinylation of a novel site of NEMO. Current Biology. 14(24): 2217-2227, 2004. 4. Costans, A. Giving a Nod2 the right target. The Scientist. 22-26, 2005. 5. Barnich, N., et al. GRIM-19 interactswith nucleatide oligomerization domain 2 and serves as downstream effector of anti-bacterial function in intestinal epithelial cells. J. of Biol. Chem. 280(19):19021-19026, 2005. 6. Berrebi, D., et al. Card 15 gene overexpression in mononuclear and epithelial cells of the inflamed Crohn's disease colon. Gut. 52:840-846, 2003. (Human, Immunohistochemistry, Immunofluoresence) 7. Chamaillard, M., et al. Gene-environment interaction modulated by allelic heterogeneity in inflammatory diseases. PNAS. 100(6): 3455-3460, 2003. (Human, Western Blot) |
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