| Catalog Code: | NB500-144 |
| Product Type: | Primary Antibody |
| Product Name: | MEK-1 (pThr386), phosphorylated Antibody |
| List Price: | 275 |
| Clonality: | Polyclonal |
| Gene ID: | 5604 |
| Host: | Rabbit |
| Product Description: | Rabbit Polyclonal anti-MEK-1 (pThr386), phosphorylated [Thr386] |
| Phosphorylated: | Thr386 |
| Cross Reactivity: | NB 500-144 reacts with human. |
| Species Summary: | Hu |
| Background: | MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in brain (Adams and |
| Alternate Names: | anti-MEK1 antibo |
| Immunogen: | NB 500-144 was raised against a Synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr386 of human MEK 1. |
| Specificity: | Specific for the ~45k MEK 1 protein phosphorylated at Thr386 in Western blots of human brain extracts. |
| Buffer: | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% glycerol |
| Packaging: | 0.1 ml affinity purified rabbit serum (affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns). *Adequate amount of material to conduct 10-mini Western Blots. |
| Package Size: | 0.1 ml |
| Uses: | NB 500-144 can be used in Dot blots and Western blot where a band is seen at ~45 kDa, representing MEK 1. Immunohistochemical analysis has not yet been determined. Suggested working dilutions: * Western/Dot Blot - 1:1,000 Immunohistochemistry - ND *The |
| Application Summary: | WB |
| Product References: | 1. Chong H, Vikis HG, Guan KL, Mechanisms of regulating the Raf kinase family. Cellular Signalling 15 (2003) 463-469. 2. Adams, J.P. and Sweatt, J.D., "Molecular psychology: Roles for the ERK MAP kinase cascade in memory." Annu. Rev. Pharmacol. |