| Catalog Code: | NB500-127 |
| Product Type: | Primary Antibody |
| Product Name: | Histone H3 Antibody |
| List Price: | 295 |
| Clonality: | Monoclonal |
| Gene ID: | 3020 |
| Host: | Mouse |
| Clone: | H3-P |
| Isotype: | IgG2a |
| Product Description: | Mouse Monoclonal anti-Histone H3 (H3-P) [Ser10] |
| Phosphorylated: | Ser10 |
| Cross Reactivity: | Human. Other species have not been tested. |
| Species Summary: | Hu |
| Background: | This antibody is a useful tool to study molecular mechanisms associated with the G2 to M transition and chromatin condensation and for the screening of in vivo inhibitors of kinase(s) or phosphatase(s) involved in H3 phosphorylation or dephosphorylation. |
| Alternate Names: | anti-H3 antibody, anti-HisH3 antibody, anti-His1H3 antibody, anti-H3/a antibody, anti-H3/b antibody, anti-H3 antibody, c antibody, anti-H3/d antibody, anti-H3/f antibody, anti-H3/h antibody, anti-H3/i antibody, anti-H3/j antibody, anti-H3/k antibody, anti |
| Immunogen: | A synthetic peptide [ARTKQTARKSpTGGKAPRK] conjugated to KLH, corresponding to amino acids 1-18 of human Histone H3. |
| Localization: | Nuclear |
| Specificity: | This antibody reacts with human histone H3 phosphorylated near its N-terminus (at Ser10) and does not detect the non-phosphorylated epitope. |
| Purity: | IgG purified |
| Buffer: | 0.01M Sodium phosphate, 0.25M sodium chloride [pH7.6], 15 mg/ml BSA |
| Packaging: | 0.1 mg IgG purified Mouse ascites. |
| Package Size: | 0.1 mg |
| Concentration: | 2 mg/ml |
| Uses: | This antibody is useful for FACS analysis, immunocytochemistry, Western blot, Dot blot, immunofluorescence, and ELISA. |
| Application Summary: | FACS, ELISA, IF, WB, ICC |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| General References: | 1. Wei, Y., et al. Phosphorylation of histone H3 is required for proper chromosome condensation and segregation. Cell 97: 99-109 (1999). 2. Chadee, D.N., et al. Increased Ser-10 phosphorylation of histone H3 in mitogen-stimulated and oncogene-transforme |
| Product References: | 1. Juan, G., et al. Histone H3 phosphorylation in human monocytes and during HL-60 cell differentiation. Exp. Cell Res. 246: 212-20 (1999). 2. Sauve, D.M., et al. Phosphorylation-induced rearrangement of the histone H3 NH2-terminal domain during mitotic |
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