| CatalogCode: | NB400-164 |
| ProductName: | ABCA1 Antibody |
| Product Description: | Rat Monoclonal anti-ABCA1 (3A1.891.3) - Astrocyte Marker |
| Clone: | 3A1.891.3 |
| Clonality: | Monoclonal |
| Immunogen: | An A1-N3 peptide (within residues 200-250) of the nouse ABCA1 protein covalently linked to chicken egg albumin by means of glutaraldehyde. |
| Epitope: | N-terminal loop |
| Specificity: | This antibody is specific for ABCA1. |
| CrossReactivity: | This antibody reacts with mouse ABCA1. It does not react with human protein. Other species have not been tested. |
| Packaging: | 0.1 ml Whole Rat ascites. |
| Uses: | NB 400-164 may be used for Western analysis, where a band is seen at ~250 kDa. This antibody does not appear to work in FACS analysis. No other applications have been tested.The investigator should determine the optimal working dilution for a specific application.**ABCA1 is known to aggregate. Do not boil samples. Use a reducing buffer: 2X sample buffer): 8M Urea, 2% SDS, 715 mM BME, 1% glycerol, 0.001% Bromophenol Blue, 125 mM Tris-HCl [pH 6.8]. |
| Localization: | Cell Membrane |
| Background: | Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG1 kappa |
| Host_Name: | Rat |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Hu(-), Mu |
| ALTnames: | anti-ABC 1 antibody; anti-ABC Transporter 1 antibody; anti-ABC1 antibod; anti-ATP-binding Cassette 1 antibody; anti-ATP-binding Cassette Transporter 1 antibody; anti-CERP antibody; anti-Cholesterol Efflux Regulatory Protein antibody; anti-ABC-1 antibody |
| ProteinTarget: | ABCA1 |
| PackageSize: | 0.1 ml |
| Marker: | Astrocyte Marker |