| CatalogCode: | | NB300-884 |
| ProductName: | | CrkL Antibody |
| Product Description: | | Goat Polyclonal anti-CrkL |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide C-KIFDPQNPDENE, representing the C-Terminus of the human protein (residues 292-303), according to NP_005198. |
| CrossReactivity: | | Reacts with Human and Mouse. Not yet tested in other species. |
| Packaging: | | 1 mg Immunogen affinity purified Goat antisera. |
| Uses: | | This antibody can be used for ELISA and Western Blot. In Western Blot, approx 37kDa band observed in K562 and Jurkat lysates (calculated MW of 33.8kDa according to NP_005198). Not tested in other applications.Recommended Starting Dilution:*ELISA (peptide): 1:16,000WB: 0.01-0.03ug/ml*Optimal dilutions should be determined by the end user. |
| Background: | | CRKL is a 33kDa adaptor protein which has been shown to activate the RAS and JUN kinase signalling pathways and transforms fibroblasts in a RAS dependant manner. CRKL is a substitute of BCR ABL tyrosine kinase. In addition, CRKL has oncogenic potential. |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Goat |
| Buffer: | | Tris-saline, [pH7.3] with 0.5% BSA |
| ListPrice: | | 295 |
| AppSummary: | | ELISA, WB |
| SpeciesSummary: | | Hu, Mu |
| ALTnames: | | anti-Crk like protein antibody, anti-Crkol antibody, anti-Oncogene CrkL antibody, anti-V crk avian sarcoma virus CT10 oncogene homolog like antibody, anti-V crk sarcoma virus CT10 oncogene homolog avian like antibody |
| ProteinTarget: | | CrkL |
| PackageSize: | | 1 mg |
| GeneralRef: | | General / background references: Senechal K et al. The CRKL adaptor protein transforms fibroblasts and functions in transformation by the BCR-ABL oncogene. J Biol Chem 271:23255-61 (1996). PubMed: 8798523. Search PubMed (MEDLINE) for references to CrkL |
| Gene_Id: | | 1399 |
| Omim_ID: | | NB110-56896 |
| more information: | | company product webpage |