| CatalogCode: | | NB300-730 |
| ProductName: | | Heat Shock Factor 1 Antibody |
| Product Description: | | Rabbit Polyclonal anti-Heat Shock Factor 1 |
| Clonality: | | Polyclonal |
| Immunogen: | | Recombinant human HSF 1 expressed in E. coli. |
| CrossReactivity: | | Cross-reacts with Human, Rat and Mouse. Not yet tested in other species. |
| Packaging: | | 0.1 ml Whole Rabbit antisera. |
| Uses: | | Western Blot: use at a dilution of 1/10,000 (ECL). By Western blot, this antibody detects an ~83 kDa protein representing HSF 1 from NIH 3T3 cell lysate. IP: Use at assay dependent dilution. GS: Use at assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Background: | | All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes. |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Whole antisera |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | PBS with 0.05% sodium azide |
| ListPrice: | | 295 |
| AppSummary: | | GS, IP, WB |
| SpeciesSummary: | | Hu, Mu, Rt |
| ALTnames: | | anti-Heat Shock Factor 1 antibody |
| ProteinTarget: | | Heat Shock Factor 1 |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | This product has been used in:Sun L et al. Activation of HSF and selective increase in heat-shock proteins by acute dexamethasone treatment. Am J Physiol Heart Circ Physiol 278:H1091-7 (2000). General / background references:Sistonen L et al. Human heat shock factors 1 and 2 are differentially activated and can synergistically induce hsp70 gene transcription. Mol Cell Biol 14:2087-99 (1994). Jurivich DA et al. Arachidonate is a potent modulator of human heat shock gene transcription. Proc Natl Acad Sci U S A 91:2280-4 (1994). Rabindran SK et al. Regulation of heat shock factor trimer formation: role of a conserved leucine zipper. Science 259:230-4 (1993). Sorger PK Heat shock factor and the heat shock response. Cell 65:363-6 (1991). Rabindran SK et al. Molecular cloning and expression of a human heat shock factor, HSF1. Proc Natl Acad Sci U S A 88:6906-10 (1991). |
| more information: | | company product webpage |