| CatalogCode: | NB300-621 |
| ProductName: | Rab9 Antibody |
| Product Description: | Mouse Monoclonal anti-Rab9 (Mab9) |
| Clone: | Mab9 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant full length protein (Dog). |
| Specificity: | Recognizes prenylated and non-prenylated rab 9. This antibody does not cross-react with other rab family members. |
| CrossReactivity: | Cross-reacts with Human, Rat, Cow, Cat, Hamster and Primate.Not yet tested in other species. |
| Packaging: | 0.1 ml Ascites Mouse ascites. |
| Uses: | Immunofluorescence: use at a dilution of 1/500 Immunohistochemistry (frozen): use at a dilution of 1/500 Immunohistochemistry (Electron microscopy): Assay dependent.Immunoprecipitation: Assay dependent.Western Blot: use at a dilution of 1/1000 Not tested in other applications.Optimal dilutions should be determined by the end user.By Western blot, this antibody detects an ~26 kDa protein representing Rab 9 from K562 cell extract. |
| Background: | Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | PBS with 0.05% sodium azide |
| ListPrice: | 295 |
| AppSummary: | IF, IHC-Fr, IP, WB |
| SpeciesSummary: | Hu, Rt, Bv, Fe, Ha, Mk |
| ProteinTarget: | Rab 9 antibody [Mab9] |
| PackageSize: | 0.1 ml |
| GeneralRef: | This product has been used in:Shapiro AD & Pfeffer SR Quantitative analysis of the interactions between prenyl Rab9, GDP dissociation inhibitor-alpha, and guanine nucleotides. J Biol Chem 270:11085-90 (1995). Soldati T et al. Rab GDI: a solubilizing and recycling factor for rab9 protein. Mol Biol Cell 4:425-34 (1993). General / background references:Schimmoller F et al. Rab GTPases, directors of vesicle docking. J Biol Chem 273:22161-4 (1998). |
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