| CatalogCode: | | NB300-620 |
| ProductName: | | NFAT2 Antibody |
| Product Description: | | Mouse Monoclonal anti-NFAT2 (7A6) |
| Clone: | | 7A6 |
| Clonality: | | Monoclonal |
| Immunogen: | | Bacterially expressed fusion protein containing NFAT 2 residues 1-654. |
| Specificity: | | Detects cytosolic nuclear factor of activated T-cells (NFAT 2) from transfected human, mouse, and monkey cells. This antibody does not cross react with NFATp. |
| CrossReactivity: | | Cross-reacts with Human, Mouse and Primate.Not yet tested in other species. |
| Packaging: | | 0.1 ml Mouse ascites. |
| Uses: | | Immunocytochemistry: use at a dilution of 1/250 Western Blot: use at a dliution of 1/2000 Gel shift: Assay dependent.Immunoprecipitation: Assay dependent.Not tested in other applicationsOptimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~105 kDa band representing transfected NFAT 2 from COS cell lysate. |
| Background: | | The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that coordinate the immune response. NFAT2 is the cytosolic component of NFAT and is encoded by a separate gene. Expression of NFAT2 activates the interleukin (IL-2) promoter in non-T lymphocytes and is required for IL-2 gene expression. |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Ascites |
| Isotype: | | IgG1 |
| Host_Name: | | Mouse |
| Buffer: | | PBS with 0.05% sodium azide |
| ListPrice: | | 295 |
| AppSummary: | | IP, WB, GS, ICC |
| SpeciesSummary: | | Hu, Mu, Mk |
| ALTnames: | | anti-MGC138448 antibody, anti-NF ATc antibody, anti-NF ATc1 antibody, anti-NFAT 2 antibody, anti-NFAT transcription complex cytosolic component antibody, anti-NFAT2 antibody, anti-NFATC 1 antibody, anti-NFATc antibody, anti-NFATc1 antibody, anti-Nuclear factor of activated T cells cytoplasmic 1 antibody, anti-Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1 antibody, anti-Nuclear factor of activated T cells cytosolic component 1 antibody |
| ProteinTarget: | | NFAT 2 antibody [7A6] |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | This product has been used in:Lyakh L et al. Expression of NFAT-family proteins in normal human T cells. Mol Cell Biol 17:2475-84 (1997). Latinis KM et al. Two NFAT transcription factor binding sites participate in the regulation of CD95 (Fas) ligand expression in activated human T cells. J Biol Chem 272:31427-34 (1997). Ho SN et al. NFATc3, a lymphoid-specific NFATc family member that is calcium-regulated and exhibits distinct DNA binding specificity. J Biol Chem 270:19898-907 (1995). Northrop JP et al. NF-AT components define a family of transcription factors targeted in T-cell activation. Nature 369:497-502 (1994). General / background references:Chow CW et al. Nuclear accumulation of NFAT4 opposed by the JNK signal transduction pathway. Science 278:1638-41 (1997). Clipstone NA et al. Molecular analysis of the interaction of calcineurin with drug-immunophilin complexes. J Biol Chem 269:26431-7 (1994). Crabtree GR Contingent genetic regulatory events in T lymphocyte activation. Science 243:355-61 (1989). Shaw JP et al. Identification of a putative regulator of early T cell activation genes. Science 241:202-5 (1988). |
| more information: | | company product webpage |