| CatalogCode: | NB300-596 |
| ProductName: | BMAL 1 Antibody |
| Product Description: | Rabbit Polyclonal anti-BMAL 1 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: CD(582)MIDNDQGSSSPS(594).Immunizing peptide corresponds to amino acid residues 582-594 from mouse BMAL 1. This sequence is completely conserved between mouse, rat, guinea pig, and human BMAL 1. |
| Specificity: | Detects BMAL 1 / aryl hydrocarbon nuclear translocator 3 (ARNT 3) from hamster tissues as well as recombinant human BMAL 1. |
| CrossReactivity: | Cross-reacts with Human, Mouse and Hamster. Not yet tested in other species. |
| Packaging: | 0.2 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | This antibody can be used in Western Blot where a 110kDa band representing hamster GST-BMAL1 fusion construct overexpressed in E. coli is seen.Recommended Starting Dilutions*:WB: 1/200Not tested in other applications.*Optimal dilutions should be determined by the end user. |
| Background: | Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS with 1 mg/ml BSA containing 0.05% sodium azide |
| ListPrice: | 295 |
| AppSummary: | WB |
| SpeciesSummary: | Ha, Hu, Mu |
| ProteinTarget: | BMAL 1 Antibody |
| PackageSize: | 0.2 ml |
| GeneralRef: | Reppert SM A clockwork explosion! Neuron 21:1-4 (1998). Darlington TK et al. Closing the circadian loop: CLOCK-induced transcription of its own inhibitors per and tim. Science 280:1599-603 (1998). |