| CatalogCode: | | NB300-587 |
| ProductName: | | Arrestin Antibody |
| Product Description: | | Rabbit Polyclonal anti-Arrestin |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic Peptide with sequence CD(384)DIVFEDFARLRLK(397)corresponding to amino acid residues 384-397 from human beta-arrestin 2. |
| Epitope: | | CDDIVFEDFARLRLK |
| Specificity: | | Detects recombinant rat beta-arrestin and beta-arrestin 2. This antibody does not detect visual or cone arrestin. |
| CrossReactivity: | | Cross-reacts with Rat. Not yet tested in other species. |
| Packaging: | | 0.2 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | | This antibody can be used in Western Blot where 49kDa and 47kDa bands are seen representing beta-arrestin and beta-arrestin 2, respectively.Recommended Starting Dilutions*:WB: 1/2000*Optimal dilutions should be determined by the end user. |
| Background: | | Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin?s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin. Arrestins are cytosolic proteins that are involved in G protein-coupled receptor (GPCR) desensitization. Arrestin binding to activated GPCRs is phosphorylation dependent and, once bound, uncouple the GPCR from the associated heterotrimeric G proteins. There are currently 4 known mammalian isoforms, beta-arrestin1 (arrestin2), beta-arrestin2 (arrestin3), visual arrestin (arrestin1), and cone arrestin. The beta- isoforms are ubiquitously expressed and are known to interact with acetylcholine and adrenergic receptors. Visual and cone arrestins are found to interact directly with transducin. |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | PBS with 1 mg/ml BSA containing 0.05% sodium azide |
| ListPrice: | | 295 |
| AppSummary: | | WB |
| SpeciesSummary: | | Rt |
| ProteinTarget: | | Arrestin (pan) Antibody |
| PackageSize: | | 0.2 mg |
| GeneralRef: | | Mundell SJ et al. Characterization of arrestin expression and function. Methods Enzymol 343:600-11 (2002). McDonald PH & Lefkowitz RJ Beta-Arrestins: new roles in regulating heptahelical receptors' functions. Cell Signal 13:683-9 (2001). Penn RB et al. Arrestin specificity for G protein-coupled receptors in human airway smooth muscle. J Biol Chem 276:32648-56 (2001). |
| more information: | | company product webpage |