| CatalogCode: | NB300-577 |
| ProductName: | Retinoid X Receptor Beta Antibody |
| Product Description: | Mouse Monoclonal anti-Retinoid X Receptor Beta (MOK13-17) |
| Clone: | MOK13-17 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant full length protein (Human). |
| Specificity: | This antibody is specific for the RXR beta isoform and does not cross react with either RXR alpha or gamma. |
| CrossReactivity: | Cross-reacts with Human and Mouse.Not yet tested in other species. |
| Packaging: | 0.1 mg Liquid |
| Uses: | Western Blot: use at a concentration of 5 ug/mlGel Shift: Assay dependent. Immunofluorescence: Assay dependent. Immunoprecipitation: Assay dependent. Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects an ~50 kDa protein representing RXR beta from transfected Sf9 cells. Immunofluorescence staining of RXR beta in MCF-7 cells overexpressing RXR beta results in strong nuclear staining. Gel super shift experiments show disruption of vitamin D receptor (VDR)/RXR beta/VDRE (DNA) complex formation when RXR beta was preincubated with this antibody, yet experiments using THR showed a supershift of the thyroid hormone receptor (THR)/RXR beta heterodimer by this product. |
| Localization: | Nuclear |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | PBS with 0.05% sodium azide |
| ListPrice: | 295 |
| AppSummary: | GS, IF, IP, WB |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-NR2B1 antibody, anti-Retinoic acid receptor RXR alpha antibody, anti-Retinoid X receptor alpha antibody, anti-RXRalpha1 antibody |
| ProteinTarget: | Retinoid X Receptor Beta antibody [MOK13-17] |
| PackageSize: | 0.1 mg |
| GeneralRef: | This product has been used in:Kephart DD et al. Retinoid X receptor isotype identity directs human vitamin D receptor heterodimer transactivation from the 24-hydroxylase vitamin D response elements in yeast. Mol Endocrinol 10:408-19 (1996). Holley SJ & Yamamoto KR A role for Hsp90 in retinoid receptor signal transduction. Mol Biol Cell 6:1833-42 (1995). Segars JH et al. Inhibition of estrogen-responsive gene activation by the retinoid X receptor beta: evidence for multiple inhibitory pathways. Mol Cell Biol 13:2258-68 (1993). MacDonald PN et al. Retinoid X receptors stimulate and 9-cis retinoic acid inhibits 1,25-dihydroxyvitamin D3-activated expression of the rat osteocalcin gene. Mol Cell Biol 13:5907-17 (1993). Hallenbeck PL et al. Heterodimerization of thyroid hormone (TH) receptor with H-2RIIBP (RXR beta) enhances DNA binding and TH-dependent transcriptional activation. Proc Natl Acad Sci U S A 89:5572-6 (1992). Marks MS et al. H-2RIIBP expressed from a baculovirus vector binds to multiple hormone response elements. Mol Endocrinol 6:219-30 (1992). Mangelsdorf DJ et al. A direct repeat in the cellular retinol-binding protein type II gene confers differential regulation by RXR and RAR. Cell 66:555-61 (1991). General / background references:Minucci S et al. Dominant negative retinoid X receptor beta inhibits retinoic acid-responsive gene regulation in embryonal carcinoma cells. Mol Cell Biol 14:360-72 (1994). Berrodin TJ et al. Heterodimerization among thyroid hormone receptor, retinoic acid receptor, retinoid X receptor, chicken ovalbumin upstream promoter transcription factor, and an endogenous liver protein. Mol Endocrinol 6:1468-78 (1992). |