| CatalogCode: | NB300-574 |
| ProductName: | Beta 1 Spectrin Antibody |
| Product Description: | Mouse Monoclonal anti-Beta 1 Spectrin (4C3) |
| Clone: | 4C3 |
| Clonality: | Monoclonal |
| Immunogen: | Purified human erythrocyte beta-1 spectrin. |
| Specificity: | Detects spectrin from erythrocytes, brain and muscle cells. This antibody has been shown to specifically detect the two known alternatively spliced forms of spectrin, beta-1 epsilon-1, present in erythrocytes, and beta-1 epsilon-2, present in nerve and striated muscle cells. It does not cross-react with alpha-2 spectrin or either of the fodrin subunits. |
| CrossReactivity: | Cross-reacts with Human, Rat and Mouse.Not yet tested in other species. |
| Packaging: | 0.1 ml Liquid |
| Uses: | Immunofluorescence: use at a dilution of 1/25 Western Blot: use at a dilution of 1/100 Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a ~246 kDa protein representing beta-1 spectrin in rat skeletal muscle homogenate. Immunofluorescence staining of beta-1 spectrin in rat skeletal muscle fibers results in intense staining of the sarcolemma.In immunofluorescence, antigen integrity can be compromised if aldehyde fixatives are left in contact with the protein for extended periods of time. If paraformaldehyde is used as a fixative, exposure should be limited to 5 minutes or less of no more than a 2% solution. |
| Background: | Spectrin (Sp), the most abundant of the erythrocyte membrane skeleton proteins, helps these cells maintain their characteristic biconcave shape while remaining flexible and elastic. Erythrocyte Sp is a heterodimer composed of a 280 kDa alpha subunit and a 246 kDa beta subunit which associate in a side-to-side, antiparallel configuration to form a 100 nm rod-like structure. Sp in other tissues may be composed of distinct but homologous alpha and beta subunits, sometimes referred to as fodrin. A newly introduced nomenclature designates the Sp subunits of the erythrocyte as alpha-1 and beta-1, and the fodrin subunits as alpha-2 and beta-2. Alternatively spliced forms of each are designated as epsilon-1, epsilon-2, etc. (e.g. beta-1 epsilon-1). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | PBS with 0.05% sodium azide |
| ListPrice: | 295 |
| AppSummary: | IF, WB |
| SpeciesSummary: | Hu, Mu, Rt |
| ProteinTarget: | Beta 1 Spectrin antibody [4C3] |
| PackageSize: | 0.1 ml |
| GeneralRef: | This product has been used in:Porter GA et al. Two populations of beta-spectrin in rat skeletal muscle. Cell Motil Cytoskeleton 37:7-19 (1997). General / background references:Dmytrenko GM et al. Dystrophin in a membrane skeletal network: localization and comparison to other proteins. J Neurosci 13:547-58 (1993). Winkelmann JC & Forget BG Erythroid and nonerythroid spectrins. Blood 81:3173-85 (1993). |