| CatalogCode: | | NB300-543 |
| ProductName: | | Ryanodine Receptor Antibody |
| Product Description: | | Mouse Monoclonal anti-Ryanodine Receptor (34C) |
| Clone: | | 34C |
| Clonality: | | Monoclonal |
| Immunogen: | | Partially purified chicken pectoral muscle ryanodine receptor. |
| Specificity: | | Detects Ryanodine Receptor (RyR)-1 and RyR-2 isoforms.In chickens, this antibody detects the alpha, beta and cardiac isoforms. This antibody detects RyR-3 in mouse cells. In frog, this antibody detects the alpha and beta isoforms. In fish, this antibody detects the alpha isoform. |
| CrossReactivity: | | Cross-reacts with Human, Rabbit, Rat, Mouse, Cow, Dog, Sheep, Fish and Amphibians. Not yet tested in other species. |
| Packaging: | | 0.1 ml Mouse ascites. |
| Uses: | | This antibody can be used in Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation, and Western Blot. By Western blot, this antibody detects a 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in chicken brain results in intense staining of cerebral Purkinje neurons.Recommended Starting Dilutions*:IHC-Fr: 1/1000WB: 1/5000Not tested in other applications.*Optimal dilutions should be determined by the end user. |
| Background: | | The Ryanodine Receptor (RyR) is the channel responsible for calcium release from muscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR. Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively. |
| ProductRef: | | Liu Z et al. Three-dimensional reconstruction of the recombinant type 3 ryanodine receptor and localization of its amino terminus. Proc Natl Acad Sci U S A 98:6104-9 (2001). Chan SL et al. Presenilin-1 mutations increase levels of ryanodine receptors and calcium release in PC12 cells and cortical neurons. J Biol Chem 275:18195-200 (2000). Yamada KA et al. Long-chain acylcarnitine induces Ca2+ efflux from the sarcoplasmic reticulum. J Cardiovasc Pharmacol 36:14-21 (2000). Moore RA et al. A transgenic myogenic cell line lacking ryanodine receptor protein for homologous expression studies: reconstitution of Ry1R protein and function. J Cell Biol 140:843-51 (1998). Protasi F et al. Role of ryanodine receptors in the assembly of calcium release units in skeletal muscle. J Cell Biol 140:831-42 (1998). Liu G & Pessah IN Molecular interaction between ryanodine receptor and glycoprotein triadin involves redox cycling of functionally important hyperreactive sulfhydryls. J Biol Chem 269:33028-34 (1994). Chen SR et al. Positioning of major tryptic fragments in the Ca2+ release channel (ryanodine receptor) resulting from partial digestion of rabbit skeletal muscle sarcoplasmic reticulum. J Biol Chem 268:22642-9 (1993). Airey JA et al. Three ryanodine receptor isoforms exist in avian striated muscles. Biochemistry 32:5739-45 (1993). Walton PD et al. Ryanodine and inositol trisphosphate receptors coexist in avian cerebellar Purkinje neurons. J Cell Biol 113:1145-57 (1991). Airey JA et al. Identification and localization of two triad junctional foot protein isoforms in mature avian fast twitch skeletal muscle. J Biol Chem 265:14187-94 (1990). General / background references:Olivares EB et al. Nonmammalian vertebrate skeletal muscles express two triad junctional foot protein isoforms. Biophys J 59:1153-63 (1991). |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Ascites |
| Isotype: | | IgG1 |
| Host_Name: | | Mouse |
| Buffer: | | PBS with 0.05% sodium azide |
| ListPrice: | | 295 |
| AppSummary: | | IHC-Fr, IP, WB |
| SpeciesSummary: | | Ca, Hu, Mu, Rt, Rb, Am, Fi, Sh |
| ProteinTarget: | | Ryanodine Receptor antibody [34C] |
| PackageSize: | | 0.1 ml |
| more information: | | company product webpage |
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