| CatalogCode: | NB300-530 |
| ProductName: | Aryl Hydrocarbon Receptor Antibody |
| Product Description: | Mouse Monoclonal anti-Aryl Hydrocarbon Receptor (RPT1) |
| Clone: | RPT1 |
| Clonality: | Monoclonal |
| Immunogen: | Synthetic Peptide: (12)RKRRKP(17)V(22)KPIPAEGIK(31). Immunizing peptide corresponds to amino acid residues 12-31 from the mouse AhR protein with amino acids 18-21 being omitted. |
| Specificity: | Detects the aryl hydrocarbon receptor (AhR). |
| CrossReactivity: | Cross-reacts with Human, Rat and Mouse.Not yet tested in other species. |
| Packaging: | 0.1 ml Mouse ascites. |
| Uses: | Western Blot: use at 1:1000 By Western blot, this antibody detects an ~95 kDa protein representing AhR from Hepa 1 cytosol. Although ab2770 can be used in immunoprecipitation procedures, clone RPT9 is recommended for this procedure. |
| Background: | The Aryl Hydrocarbon Receptor (AHR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the AH Receptor. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AHR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AHR followed by formation of a heterodimer with AHR nuclear translocator protein (Arnt). The AHR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Ascitic fluid |
| ListPrice: | 295 |
| AppSummary: | IP, WB |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-Dioxin Receptor antibody |
| ProteinTarget: | Aryl Hydrocarbon Receptor [RPT1] Antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | This product has been used in:Ray SS & Swanson HI Dioxin-induced immortalization of normal human keratinocytes and silencing of p53 and p16INK4a. J Biol Chem 279:27187-93 (2004). General / background references:Delescluse C et al. Is CYP1A1 induction always related to AHR signaling pathway? Toxicology 153:73-82 (2000). Perdew GH et al. Production and characterization of monoclonal antibodies directed against the Ah receptor. Hybridoma 14:279-83 (1995). Chen HS & Perdew GH Subunit composition of the heteromeric cytosolic aryl hydrocarbon receptor complex. J Biol Chem 269:27554-8 (1994). |