| CatalogCode: | | NB300-521 |
| ProductName: | | COP II Antibody |
| Product Description: | | Rabbit Polyclonal anti-COP II |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide: M(1)TTYLEFIQQNEERDGVR(18) C. Immunizing peptide corresponds to amino acid residues 1-18 from rat COPII (SEC 23). This sequence is completely conserved between human, mouse, and rat. |
| CrossReactivity: | | Cross-reacts with Human, Rat and Mouse. |
| Packaging: | | 0.1 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | | Western blot, this antibody detects an ~85 kDa protein representing COPII from PC-12 cell extract. Due to a customer feedback we no longer recommend this product for ICC. |
| Background: | | Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two Coatomer-Protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits. |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | PBS, 1 mg/ml BSA, 0.05% sodium azide |
| ListPrice: | | 295 |
| AppSummary: | | ICC, WB |
| SpeciesSummary: | | Hu, Mu, Rt |
| ProteinTarget: | | COP II |
| PackageSize: | | 0.1 mg |
| GeneralRef: | | General / background references:Futatsumori M et al. Identification and characterization of novel isoforms of COP I subunits. J Biochem (Tokyo) 128:793-801 (2000). Shimoni Y et al. Lst1p and Sec24p cooperate in sorting of the plasma membrane ATPase into COPII vesicles in Saccharomyces cerevisiae. J Cell Biol 151:973-84 (2000). Barlowe C Traffic COPs of the early secretory pathway. Traffic 1:371-7 (2000). |
| more information: | | company product webpage |
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