| CatalogCode: | NB300-517 |
| ProductName: | PDI Antibody |
| Product Description: | Mouse Monoclonal anti-PDI (RL90) |
| Clone: | RL90 |
| Clonality: | Monoclonal |
| Immunogen: | Full length native protein (purified) (Rat). |
| CrossReactivity: | Cross-reacts with Human, Rat, Mouse and Hamster. Not yet tested in other species. |
| Packaging: | 0.1 mg diluted ascites. |
| Uses: | This antibody can be used in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blot. By Western blot, this antibody detects a protein at 59 kDa representing PDI from rat liver extract or a slightly higher protein at 61 kDa representing PDI from human liver extract.Immunohistochemical staining of PDI in rat intestine yields a pattern consistent with cytoplasmic staining.In immunoprecipitation procedures, this antibody has been shown to inhibit the activity of PDI in vitro. This antibody has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells.Recommended Starting Dilutions*:IF: 1/100 IHC: 1/100 WB: 1/1000 Not tested in other applications.*Optimal dilutions should be determined by the end user. |
| Background: | The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide-Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. |
| ProductRef: | Lahav J et al. Sustained integrin ligation involves extracellular free sulfhydryls and enzymatically catalyzed disulfide exchange. Blood 100:2472-8 (2002). PUBMED:12239158 Tanaka S et al. Up-regulation of protein-disulfide isomerase in response to hypoxia/brain ischemia and its protective effect against apoptotic cell death. J Biol Chem 275:10388-93 (2000). PUBMED:10744727 Bennett TA et al. Sulfhydryl regulation of L-selectin shedding: phenylarsine oxide promotes activation-independent L-selectin shedding from leukocytes. J Immunol 164:4120-9 (2000). PUBMED:10754306 Courageot J et al. Intracellular degradation of the HIV-1 envelope glycoprotein. Evidence for, and some characteristics of, an endoplasmic reticulum degradation pathway. Eur J Biochem 260:482-9 (1999). PUBMED:10095785 Kaetzel CS et al. Protein disulphide-isomerase from human placenta and rat liver. Purification and immunological characterization with monoclonal antibodies. Biochem J 241:39-47 (1987). PUBMED:3566712 |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG2a |
| Host_Name: | Mouse |
| Buffer: | PBS with 0.05% sodium azide |
| ListPrice: | 295 |
| NovusRef: | 1. Kim SY, Jun HS, Mead PA, Mansfield BC, Chou JY. Neutrophil stress and apoptosis underlie myeloid dysfunction in glycogen storage disease type Ib. Blood. April 17, 2008:blood-2007-12-129114. |
| AppSummary: | IF, IHC-Fr, IHC-P, IP, WB |
| SpeciesSummary: | Ha, Hu, Mu, Rt |
| ALTnames: | anti-Disulphide Isomerase antibody, anti-GIT antibody, anti-Glutathione Insulin Transhydrogenase antibody, anti-Procollagen Proline 2 Oxoglutarate 4 Dioxygenase beta antibody, anti-Protein Disulphide Isomerase antibody, anti-Thyroid Hormone Binding Protein p55 antibody |
| ProteinTarget: | PDI antibody [RL90] |
| PackageSize: | 0.1 ml |
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