| CatalogCode: | NB300-514 |
| ProductName: | Mannose 6 Phosphate Receptor Antibody |
| Product Description: | Mouse Monoclonal anti-Mannose 6 Phosphate Receptor (2G11) |
| Clone: | 2G11 |
| Clonality: | Monoclonal |
| Immunogen: | Purified bovine 300 kDa CI-MPR |
| Epitope: | This antibody is shown to recognize an epitope in the extracellular domain of MPR. |
| CrossReactivity: | Cross-reacts with Human, Cow and Non Human Primates. Not yet tested in other species. |
| Packaging: | 0.1 mg IgG purified Mouse ascites. |
| Uses: | This antibody can be used in Immunofluorescence, Immunoprecipitation, and Western Blot. By Western Blot, a 300 kDa protein representing MPR is seen in HeLa cell extract under non-reducing conditions. Immunofluorescence staining of MPR in HeLa cells with this antibody results in perinuclear staining.Recommended Starting Dilutions*:IHC: 2-5 ug/mlWB: 1 ug/ml*Optimal dilutions should be determined by the end user. |
| Background: | Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of 300 kDa cation independent mannose 6-phospate receptors (CI-MPR). The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans Golgi network to late endosomes or early lysosomes. The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes. The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady state distribution of MPR's is predominantly within late endosomes, fewer in the trans Golgi network and ~10 % at cell surface. In addition to its man6P binding activity, the MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously. An ~240 kDa soluble, truncated form, representing the extracellular domain of the protein, has also been found circulating in serum and is capable of binding both ligands. |
| ProductRef: | Dintzis SM et al. Receptor extracellular domains may contain trafficking information. Studies of the 300-kDa mannose 6-phosphate receptor. J Biol Chem 269:12159-66 (1994). |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG2a |
| Host_Name: | Mouse |
| Buffer: | PBS with 0.05% sodium azide |
| ListPrice: | 295 |
| AppSummary: | IF, IP, WB |
| SpeciesSummary: | Bv, Hu, Mk |
| ProteinTarget: | Mannose 6-Phosphate Receptor antibody [2G11] |
| PackageSize: | 0.1 mg |
| GeneralRef: | MacDonald RG et al. Serum form of the rat insulin-like growth factor II/mannose 6-phosphate receptor is truncated in the carboxyl-terminal domain. J Biol Chem 264:3256-61 (1989). |