| CatalogCode: | | NB300-510 |
| ProductName: | | VAMP3 Antibody |
| Product Description: | | Rabbit Polyclonal anti-VAMP3 |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide with sequence STGVPSGSSAATGSNRRC, corresponding to amino acids 1-18 of Mouse VAMP3. |
| Epitope: | | STGVPSGSSAATGSNRRC |
| CrossReactivity: | | Cross-reacts with Dog, Human, Mouse and Rat. Not yet tested in other species. |
| Packaging: | | 0.1 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | | This antibody can be used in Immunofluorescence and Western Blot. By Western Blot a 13kDa band is seen (predicted MW 12kDa).Recommended Starting Dilutions*:WB: 2 ug/mlNot tested in other applications.*Optimal dilutions should be determined by the end user. |
| Background: | | The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer?s disease, all VAMP isoform levels appear to be significantly reduced. |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | PBS with 1mg/ml BSA containing 0.05% Sodium azide |
| ListPrice: | | 295 |
| NovusRef: | | 1. Tiwari N, Wang C-C, Brochetta C, et al. VAMP-8 segregates mast cell-preformed mediator exocytosis from cytokine trafficking pathways. Blood. April 1, 2008;111(7):3665-74. 2. Williams D, Pessin JE. Mapping of R-SNARE function at distinct intracellular GLUT4 trafficking steps in adipocytes. J Cell Biol. 2008 January 28, 2008;180(2):375-87. |
| AppSummary: | | IF, WB |
| SpeciesSummary: | | Ca, Hu, Mu, Rt |
| ProteinTarget: | | VAMP3 antibody |
| PackageSize: | | 0.1 mg |
| GeneralRef: | | Annaert WG et al. Export of cellubrevin from the endoplasmic reticulum is controlled by BAP31. J Cell Biol 139:1397-410 (1997). McMahon HT et al. Cellubrevin is a ubiquitous tetanus-toxin substrate homologous to a putative synaptic vesicle fusion protein. Nature 364:346-9 (1993). |
| more information: | | company product webpage |