| CatalogCode: | NB300-507 |
| ProductName: | Parkin Antibody |
| Product Description: | Rabbit Polyclonal anti-Parkin |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide RILGEEQYNRYQQYGAEEC corresponds to aa 305-323 of the human parkin protein. The peptide conjugated to diphtheria toxoid cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC). |
| Specificity: | An internal peptide sequence was chosen to avoid possible cross reactivity with ubiquitin. |
| CrossReactivity: | Cross-reacts with Human and Rat. Not yet tested in other species. |
| Packaging: | 0.1 ml purified antisera. |
| Uses: | This antibody can be used in Western Blot and Immunohistochemistry. By Western Blot a 50kDa band is seen in rat brain.Recommended Starting Dilutions*:IHC: 1/1,000WB: 0.5ug/ml*Optimal dilutions should be determined by the end user. |
| Control: | Rat brain extract (ref. 1) |
| Background: | Parkinson's disease is a common neurodegenerative disease with complex clinical features. Mutations in the gene, Parkin (PARK2), appear to be responsible for the pathogenesis of autosomal recessive juvenile Parkinsonism. Parkin plays a role in the ubiquitin-mediated proteolytic pathway by removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of Parkin. Parkin may protect neurons against alpha synuclein toxicity, proteasomal dysfunction, gpr37 accumulation, and kainate-induced excitotoxicity. It may play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Parkin also regulates cyclin e during neuronal apoptosis and may represent a tumor suppressor gene. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS with 1 mg/ml BSA containing 0.05% sodium azide |
| ListPrice: | 295 |
| AppSummary: | IHC-Fr, IHC-P, WB |
| SpeciesSummary: | Hu, Rt |
| ALTnames: | anti-Ubiquitin E3 ligase PRKN antibody, anti-Parkinson juvenile disease protein 2 antibody, anti-Parkinson disease protein 2. GENE NAME: PARK2 antibody, anti-PRKN antibody |
| ProteinTarget: | Parkin |
| PackageSize: | 0.1 ml |
| GeneralRef: | Kitada T et al. Mutations in the parkin gene cause autosomal recessive juvenile parkinsonism. Nature 392:605-8 (1998). |
| NotesMain: | This antibody stains trigeminal motor neurons in rat brain stem. |
order or
more information: | company product webpage |
| request information: |
|