| CatalogCode: | NB300-506 |
| ProductName: | GnRH Antibody |
| Product Description: | Rabbit Polyclonal anti-GnRH |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: EHWSYGL RPG (Human). |
| CrossReactivity: | Cross-reacts with Human and Mouse. Expected to cross-react with Hamster (90% identity with immunogen), Pig (90% identity with immunogen), Rat (90% identity with immunogen), Rhesus monkey (90% identity with immunogen), Sheep (90% identity with immunogen), Xenopus laevis (90% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 ml Affinity purified Rabbit antisera. |
| Uses: | This antibody can be used in Immunofluorescence and Immunohistochemistry. Immunofluorescent staining of GnRH in mouse embryo brain tissue results in staining of neurons in the rostral forebrain. In immunohistochemical procedures, 4% paraformaldehyde is recommended as a fixative. Recommended Starting Dilutions*:IHC: 1 ug/ml Not tested in other applications.*Optimal dilutions should be determined by the end user. |
| Control: | Mouse brain tissue sections. |
| Background: | Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle.Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS with 1 mg/ml BSA containing 0.05% sodium azide |
| ListPrice: | 295 |
| AppSummary: | IF, IHC-Fr |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-Gonadotropin releasing hormone antibody, anti-LHRH antibody, anti-Luteinizing Hormorne Releasing Hormone antibody |
| ProteinTarget: | GnRH antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | Norwitz ER et al. Activin A augments GnRH-mediated transcriptional activation of the mouse GnRH receptor gene. Endocrinology 143:985-97 (2002). Kennedy SR et al. Growth hormone response to a novel growth hormone-releasing tripeptide in horses: interaction with gonadotropin-releasing hormone, thyrotropin-releasing hormone, and sulpiride. J Anim Sci 80:744-50 (2002). |
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