| CatalogCode: | NB300-503 |
| ProductName: | CRM 1 Antibody |
| Product Description: | Rabbit Polyclonal anti-CRM 1 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic Peptide: CR(1043)QADEEKHKRQMSVPGS(1058).Synthetic peptide corresponds to amino acid residues 1043-1058 from human CRM 1 protein. This sequence is 88% conserved in mouse, rat and Xenopus laevis. |
| CrossReactivity: | Cross-reacts with Human and Cow. Expected to cross-react with Rat, Mouse and Xenopus laevis due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | This antibody can be used in Immunoprecipitation and Western Blot. By Western blot, this antibody detects an ~135 kDa protein representing CRM 1 from bovine retinal extract.Recommended Starting Dilutions*:WB: 0.4 ug/mlNot tested in other applications.*Optimal dilutions should be determined by the end user. |
| Background: | Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPCs. NPCs contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Active transport of molecules through the NPC is a signal-mediated process that is not well understood. Proteins to be exported from the nucleus to cytoplasm contain leucine-rich nuclear export signals (NESs). NESs are recognized by transport receptors belonging to the importin-beta family of proteins. It has been shown that NES export is mediated by CRM 1 (Chromosome Region Maintenance 1), an importin-beta family member that binds NESs, Ran:GTP, and Ran binding protein (RanBP). CRM 1 mediates the nuclear exportation of numerous proteins, including histone deacetylases (HDACs), viral proteins, immunophilins, and STAT 1. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS with 1 mg/ml BSA containing 0.05% sodium azide |
| ListPrice: | 295 |
| AppSummary: | IP, WB |
| SpeciesSummary: | Bv, Hu |
| ProteinTarget: | CRM 1 Antibody |
| PackageSize: | 0.1 mg |
| GeneralRef: | Lindsay ME et al. Ran-binding protein 3 is a cofactor for Crm1-mediated nuclear protein export. J Cell Biol 153:1391-402 (2001). Heger P et al. Qualitative highly divergent nuclear export signals can regulate export by the competition for transport cofactors in vivo. Traffic 2:544-55 (2001). Jensen TH et al. Identification of novel Saccharomyces cerevisiae proteins with nuclear export activity: cell cycle-regulated transcription factor ace2p shows cell cycle-independent nucleocytoplasmic shuttling. Mol Cell Biol 20:8047-58 (2000). |