| CatalogCode: | NB300-500 |
| ProductName: | eNOS Antibody |
| Product Description: | Rabbit Polyclonal anti-eNOS |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide with sequence SLQERQLRGAVPWAFD, corresponding to amino acids 1179-1194 of Human NOS (eNOS). |
| Epitope: | SLQERQLRGAVPWAFD |
| Specificity: | This antibody does not detect human inducible NOS or rat brain NOS. |
| CrossReactivity: | Cross-reacts with Cow, Dog, Human, Mouse, Pig and Rat.Expected to cross-react with Guinea pig (93% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | This antibody can be used in Western Blot and Immunohistochemistry. In IHC, antigen retrieval is not essential but may optimise staining. By Western Blot, a 140kDa band is seen in human endothelial cells.Recommended Starting Dilutions:IHC-P: 1/100WB: 1/1000 Not tested in other applications.*Optimal dilutions should be determined by the end user. |
| Control: | Human endothelial cells. |
| Background: | Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS with 1mg/ml BSA |
| ListPrice: | 295 |
| AppSummary: | IHC-P, WB |
| SpeciesSummary: | Bv, Ca, Hu, Mu, Po, Rt |
| ProteinTarget: | eNOS antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | Tomikawa M et al. Decreased endothelial nitric oxide synthase in gastric mucosa of rats with chronic renal failure. Am J Physiol 274:F1102-8 (1998). Stefanovic-Racic M et al. Nitric oxide and arthritis. Arthritis Rheum 36:1036-44 (1993). |
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