| CatalogCode: | | NB300-367 |
| ProductName: | | MDM2 Antibody |
| Product Description: | | Chicken Polyclonal anti-MDM2 |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide: corresponding to amino acids 28-213 of MDM2. |
| CrossReactivity: | | Cross-reacts with Human. Expected to cross-react with Mouse (based on protein sequence homology). Not yet tested in other species. |
| Packaging: | | 0.05 mg Immunogen affinity purified Chicken antisera. |
| Uses: | | WB: Use at a dilution of 1/2000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Background: | | MDM2 is a nuclear phosphoprotein with an apparent molecular mass of 90 kD that forms a complex with the p53 tumor suppressor protein. Human MDM2 was identified as a homologous product of the 'murine double minute 2' gene (mdm2). The MDM2 gene enhances the tumorigenic potential of cells when it is overexpressed and encodes a putative transcription factor. Forming a tight complex with the p53 gene, the MDM2 oncogene can inhibit p53 mediated transactivation, MDM2 also binds to p53 protein. Inactivation of tumor suppressor genes leads to deregulated cell proliferation and is a key factor in human tumorigenesis. p53 can be subjected to negative regulation by the product of a single cellular protooncogene. The interference of binding to p53 prevents the interaction of MDM2 and its regulation of the transcriptional activity of p53 in vivo. Direct association of p53 with the cellular protein MDM2 results in ubiquitination and subsequent degradation of p53. MDM2 p53 complexes were preferentially found in S/G2M phases of the cell cycle. The MDM2 gene is alternatively spliced, producing 5 additional splice variant transcripts from the full length MDM2 gene. The alternatively spliced transcripts tend to be expressed in tumorigenic tissue, whereas the full length MDM2 transcript is expressed in normal tissue. |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgY |
| Host_Name: | | Chicken |
| Buffer: | | PBS |
| ListPrice: | | 295 |
| AppSummary: | | WB |
| SpeciesSummary: | | Hu |
| ALTnames: | | anti-MDM2 antibody, anti-Mdm2 antibody, anti-transformed 3T3 cell double minute 2 antibody, anti-p53 binding protein (mouse) antibody, anti-HGNC:6973 antibody, anti-MGC71221 antibody, anti-hdm2 antibody, anti-mouse double minute 2 homolog antibody, anti-mouse double minute 2 antibody, anti-human homolog of antibody, anti-p53-binding protein antibody, anti-p53-binding protein MDM2 antibody, anti-ubiquitin-protein ligase E3 Mdm2 antibody |
| ProteinTarget: | | MDM2 antibody |
| PackageSize: | | 0.05 mg |
| GeneralRef: | | 1. Pawelek PD, MacKenzie RE, Methenyltetrahydrofolate cyclohydrolase is rate limiting for the enzymatic conversion of 10-formyltetrahydrofolate to 5,10-methylenetetrahydrofolate in bifunctional dehydrogenase-cyclohydrolase enzymes. Biochemistry. 1998 Jan 27;37(4):1109-15. 2. Kokame K, Kato H, Miyata T, Homocysteine-respondent genes in vascular endothelial cells identified by differential display analysis. GRP78/BiP and novel genes. J Biol Chem. 1996 Nov 22;271(47):29659-65. 3. Yang XM, MacKenzie RE, NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase is the mammalian homolog of the mitochondrial enzyme encoded by the yeast MIS1 gene. Biochemistry. 1993 Oct 19;32(41):11118-23. 4. Peri KG, Belanger C, Mackenzie RE, Nucleotide sequence of the human NAD-dependent methylene tetrahydrofolate dehydrogenase-cyclohydrolase. Nucleic Acids Res. 1989 Nov 11;17(21):8853 |
| more information: | | company product webpage |
|