| CatalogCode: | NB300-354 |
| ProductName: | Cdc25B Antibody |
| Product Description: | Chicken Polyclonal anti-Cdc25B |
| Clonality: | Polyclonal |
| Immunogen: | Recombinant fragment, corresponding to amino acids 140-185 of Human Cdc25B. |
| CrossReactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Chicken antisera. |
| Uses: | ICC: Use at a dilution of 1/200. WB: Use at a dilution of 1/2000. Predicted molecular weight: 65 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Background: | The activity of cyclin dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and Cdc25 phosphatases. Three Cdc25 genes are present in human cells: Cdc25A, Cdc25B and Cdc25C. Cdc25A and Cdc25B are expressed throughout the cell cycle, with peak expression in G1 for Cdc25A and in both G1 S phase and G2 for Cdc25B. Cdc25C is mainly expressed in G2 phase. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgY |
| Host_Name: | Chicken |
| Buffer: | PBS |
| ListPrice: | 285 |
| AppSummary: | WB, ICC |
| SpeciesSummary: | Hu |
| ALTnames: | anti-M-phase inducer phosphatase 2 antibody |
| ProteinTarget: | Cdc25B antibody |
| PackageSize: | 0.05 mg |
| GeneralRef: | General / background references:Lincoln AJ et al. Cdc25b phosphatase is required for resumption of meiosis during oocyte maturation. Nat Genet 30:446-9 (2002). |