| Catalog Code: | NB300-323 |
| Product Type: | Primary Antibody |
| Product Name: | GAPDH Antibody |
| List Price: | 295 |
| Clonality: | Polyclonal |
| Gene ID: | 2597 |
| Host: | Rabbit |
| Product Description: | Rabbit Polyclonal anti-GAPDH |
| Cross Reactivity: | Human and mouse. Other species have not been tested. Reactivity with X. laevis and S. Pombe and S. cerevisae GAPDH is expected owing to conservation of sequence between this ortholog and human GAPDH at the site to which the epitope maps. |
| Species Summary: | Hu, Mu, Ye |
| Background: | GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehy |
| Alternate Names: | anti-GAPDH antibody, anti-glyceraldehyde-3-phosphate dehydrogenase antibody, anti-HGNC:4141 antibody, anti-G3PD antibody, anti-GAPD antibody, anti-MGC88685 antibody |
| Immunogen: | The epitope recognized by NB 300-322 maps to a region between residues 200 and 250 of human Glyceraldehyde-3-Phosphate Dehydrogenase using the numbering given in entry NP_002037.2 (GeneID 2597). |
| Buffer: | TBS with 0.1% BSA containing 0.09% sodium azide |
| Packaging: | 0.1 ml of affinity purified rabbit sera in a Tris-citrate/phosphate (pH 7-8) buffer with 0.1% sodium azide. |
| Package Size: | 0.1 ml |
| Concentration: | 0.2 mg/ml |
| Uses: | Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows.* Western Blot: 1:2,000 - 1:10,000 Immunoprecipitation: not recommended IHC/ICC |
| Application Summary: | WB |