| CatalogCode: | NB300-321 |
| ProductName: | CASK Antibody |
| Product Description: | Rabbit Polyclonal anti-CASK |
| Clonality: | Polyclonal |
| Immunogen: | Peptide corresponding to amino acid residues 578-592 from human CASK. This sequence is completely conserved between rat, mouse, and human CASK. |
| Epitope: | The epitope lies between amino acid residues 578-592 from human CASK. |
| Specificity: | By Western blot, this antibody detects a 112 kDa protein representing CASK from AtT20 cell lysate. Immunohistochemical staining of CASK in mouse brain with this antibody results in staining of the choroid plexus. |
| CrossReactivity: | Cross-reacts with Human, Rat and Mouse. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | Immunohistochemistry: assay dependent. Western Blot: use at a concentration of 1ug/ml. Not tested in other applications. Optimal dilutions should be determined by the end user. |
| Localization: | Cytoplasmic (Potential). |
| Control: | WB: AtT20 cell lysate; IHC: mouse brain |
| Background: | Calmodulin sensitive kinase (CASK/mLIN-2) is a ~112-kDa member of the membrane associated quanylate kinase (MAGUK) protein family with multiple protein binding domains. These include an N-terminal calmodulin kinase (CaMKII) domain and C-terminal PDZ, SH3-, and quanylate kinase domains. CASK possesses both tyrosine kinase and serine/threonine kinase activity. In addition, CASK can form a heterotrimeric complex with Mint (X11or mLIN-10) and Veli (mLIN-7) proteins which is predicted to participate in plasma membrane receptor localization. CASK is localized to neuronal synapses and also interacts with syndecan and neurexin. The guanylate kinase domain of CASK has been shown to interact with the T-box transcription factor, Tbr-1, as a coactivator to induce transcription of genes that contain T-elements. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS, 1 mg/ml BSA |
| ListPrice: | 295 |
| AppSummary: | IHC, WB |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | Anti-Calmodulin sensitive kinase Calcium/calmodulin dependent serine protein kinase membrane associated guanylate kinase antibody, anti- Calcium/Calmodulin-dependent Serine Protein Kinase antibody, anti- CAMGUK protein antibody, anti- hCASK antibody, anti-Lin 2 homolog antibody, anti-LIN2 antibody, anti- Lin2 homolog antibody, anti- Peripheral plasma membrane protein CASK antibody antibody |
| ProteinTarget: | CASK Antibody |
| PackageSize: | 0.1 mg |
| GeneralRef: | Martinez-Estrada OM et al. Association of junctional adhesion molecule with calcium/calmodulin-dependent serine protein kinase (CASK/LIN-2) in human epithelial caco-2 cells. J Biol Chem 276:9291-6 (2001). Hata Y et al. Synaptic PDZ domain-containing proteins. Neurosci Res 32:1-7 (1998). |