| CatalogCode: | NB300-255 |
| ProductName: | Dopamine Transporter, Extracellular Loop 2 Antibody |
| Product Description: | Rabbit Polyclonal anti-Dopamine Transporter, Extracellular Loop 2 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide from EL2 region of human Dopamine Transporter (DAT); conjugated to keyhole limpet hemocyanin (KLH). |
| Specificity: | Specific labeling in Western blot applications with SDS-solubilized human striatal samples and in immunohistochemical applications with formaldehyde-fixed human and monkey brain sections. |
| CrossReactivity: | This antibody recognizes DAT in human and monkey striatum. |
| Packaging: | 0.1 ml in 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg per ml BSA and 50% glycerol. Adequate amount of material to conduct at least 10-mini Western Blots. Prepared from rabbit serum by affinity purification using a peptide-SulfoLink |
| Background: | Plasmalemmal neurotransmitter transporters sequester synaptic and peri-synaptic transmitter into presynaptic elements. The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. Levels of DAT protein expression are altered by chronic drug administration. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Host_Name: | Rabbit |
| ListPrice: | 295 |
| AppSummary: | WB, IHC-Fr |
| SpeciesSummary: | Hu, Mk |
| ProteinTarget: | Dopamine Transporter, Extracellular Loop 2 Antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Erickson, J.D., Schafer, M.K.H., Bonner, T.I., Eiden, L.E. and Weihe, E. "Distinct pharmacological properties and distribution in neurons and endocrine cells of two isoforms of the human vesicular monoamine transporter," Proc. Natl. Acad. Sci. USA 93 (1996) 5166 - 5171. 2. Giros, B., El Mestikawy, S., Godinot, N., Zheng, K., Han, H., Yang-Feng, T. and Caron, M.LG. "Cloning, pharmacological characterization, and chromosome assignment of the human dopamine transporter," J. Pharmacol. Exptl. Ther. 56 (1991) 2139 - 2142. 3. Haycock, J.W., Becker, L., Ang, L., Furukawa, Y., Hornykiewicz, O. and Kish, S.J. "Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum," J. Neurochem. 87 (2003) 574 - 585. 4. Wilson, J.M., Kalasinsky, K.S., Levey, A.I., Bergeron, C., Reiber, G., Anthony, R.M., Schmunk, G.A., Shannak, K., Haycock, J.W. and Kish, S.J. "Striatal dopamine nerve terminal markers in human, chronic methamphetamine users," Nature Medicine 2 (1996) 699 - 703. |
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