| CatalogCode: | NB300-253 |
| ProductName: | DOPA Decarboxylase Antibody |
| Product Description: | Sheep Polyclonal anti-DOPA Decarboxylase |
| Clonality: | Polyclonal |
| Immunogen: | SDS denatured recombinant bovine DOPA Decarboxylase (DDC) expressed in E. coli and purified from inclusion bodies. |
| Specificity: | The affinity-purified antibody has been tested using blot immunolabeling procedures, in which a single MW ~55kDa band is detected. |
| CrossReactivity: | This antibody recognizes DDC in bovine, canine, guinea pig, human, porcine, rat and sheep adrenal medulla and caudate. |
| Packaging: | 0.1 ml Immunogen affinity purified Sheep antisera. |
| Uses: | Western Blot. |
| Background: | DOPA Decarboxylase (aromatic L-amino acid decarboxylase, AADC; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin (Waymire and Haycock, 2002; Berry et al., 1996; Haycock et al., 2003). It is also involved in the biosynthesis of trace amines. DDC antibodies can therefore be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Host_Name: | Sheep |
| Buffer: | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg per ml BSA and 50% glycerol |
| ListPrice: | 295 |
| AppSummary: | WB |
| SpeciesSummary: | Bv, Ca, Hu, Po, Rt, Gp, Sh |
| ALTnames: | anti-DDC antibody, anti-dopa decarboxylase (aromatic L-amino acid decarboxylase) antibody, anti-HGNC antibody, 2719 antibody, anti-AADC antibody, anti-aromatic L-amino acid decarboxylase antibody |
| ProteinTarget: | DOPA Decarboxylase antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Berry MD, Juorio AV, Li XM, Boulton AA (1996) Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme. Neurochem Res 21:1075-1087. 2. Haycock JW, Becker L, Ang L, Furukawa Y, Hornykiewicz O, Kish SJ (2003) Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum. J Neurochem 87:574-585. 3. Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567. 4. Waymire JC, Haycock JW (2002) Lack of regulation of aromatic L-amino acid decarboxylase in intact bovine chromaffin cells. J Neurochem 81:589-593. 5. Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44. 6. Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286. |
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