| Catalog Code: | NB300-234 |
| Product Type: | Primary Antibody |
| Product Name: | DARPP-32 Antibody |
| List Price: | 275 |
| Clonality: | Polyclonal |
| Gene ID: | 84152 |
| Host: | Rabbit |
| Product Description: | Rabbit Polyclonal anti-DARPP-32 [Thr75] |
| Phosphorylated: | Thr75 |
| Cross Reactivity: | This antibody recognizes human, mouse, and rat forms of the protein. Other species have not been tested. |
| Species Summary: | Hu, Mu, Rt |
| Background: | DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DAR |
| Alternate Names: | anti-DARPP 32 antibody, anti-DARPP32 antibody, anti-Dopamine- and cAMP-regulated neuronal phosphoprotein antibody |
| Immunogen: | Synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr75 of DARPP-32. The sequence of the immunogen is identical in human and mouse. |
| Specificity: | Specific for the ~34k DARPP-32 phosphorylated at Thr75. |
| Purity: | Affinity purified |
| Packaging: | 0.1 ml affinity purified antibody in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
| Package Size: | 0.1 ml |
| Uses: | Western and Dot blots. Suggested working dilutions: * Immunoblot - 1:1,000 Immunohistochemistry - ND *The investigator should determine the optimal working dilution for a specific application. |
| Application Summary: | WB, DB |
| Product References: | 1. Svenningsson, P., Tzavara, E.T., Carruthers, R., Rachleff, I., Wattler, S., Nehls, M., McKinzie, D.L., Fienberg, A.A., Nomikos, G.G.and Greengard, P., "Diverse psychotomimetics act through a common signaling pathway," Science, 302 (2003) 1412 |
| Novus References: | Han, et al. Spatial targeting of type II protein kinase A to filopodia mediates the regu...J. Cell Biol. 176:101, 2007. |
| Notes Main: | Stock solutions are stable for a minimum of 1 year at -20 degrees Celcius. |
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