| Catalog Code: | NB300-233 |
| Product Type: | Primary Antibody |
| Product Name: | TAO-2 (pSer181), phosphorylated Antibody |
| List Price: | 275 |
| Clonality: | Polyclonal |
| Gene ID: | 9344 |
| Host: | Rabbit |
| Product Description: | Rabbit Polyclonal anti-TAO-2 (pSer181), phosphorylated [Ser181] |
| Phosphorylated: | Ser181 |
| Cross Reactivity: | This antibody recognizes the rat form of the protein. Other species have not been tested. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. |
| Species Summary: | Rt |
| Background: | In vitro, TAO (thousand and one amino acid) protein kinase 2 (TAO2) activates MAP/ERK kinases (MEKs) 3, 4, and 6 toward their substrates p38 MAP kinase JNK/SAPK (Chen et al., 1999; Chen and Cobb, 2001). This and more recent work has led to the proposal t |
| Alternate Names: | anti-TAO2 antibody |
| Immunogen: | Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho Ser181 of TAO2. |
| Specificity: | Specific for the ~120k TAO2 phosphorylated at Ser181 in Western blots of rat hippocampal lysate. |
| Purity: | Affinity purified |
| Buffer: | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg per ml BSA and 50% glycerol |
| Packaging: | 0.1 ml affinity purified rabbit serum (affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns). *Adequate amount of material to conduct 10-mini Western Blots. |
| Package Size: | 0.1 ml |
| Uses: | Western and Dot blots. Suggested working dilutions: * Immunoblot - 1:1,000 Immunohistochemistry - ND *The investigator should determine the optimal working dilution for a specific application. |
| Application Summary: | WB, DB |
| Product References: | 1. Chen, Z., Raman, M., Chen, L., Lee, S.F., Gilman, A.G., and Cobb, M.H., "TAO (thousand-and-one amino acid) protein kinases mediate signaling from carbachol to p38 mitogen-activated protein kinase and ternary complex factors," J. Biol. Chem. 2 |
| Notes Main: | Stock solutions are stable for a minimum of 1 year at -20 degrees Celcius. |