| CatalogCode: | | NB300-224 |
| ProductName: | | DARPP-32 Antibody |
| Product Description: | | Rabbit Polyclonal anti-DARPP-32 [Thr34] - Neuronal Marker |
| Clonality: | | Polyclonal |
| Immunogen: | | The immunogen used to generate this antibody was a synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho Thr34 of DARPP-32. |
| CrossReactivity: | | Human, mouse, and rat. Expected to react with bovine, chicken, canine and non-human primates due to 100% sequence homology. Other species have not been tested. |
| Packaging: | | 0.1 ml Rabbit antisera. |
| Uses: | | This antibody is useful for Western blot, where a band is seen at approximately 32 kDa. |
| Control: | | Rat caudate lysates |
| Background: | | DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine, and Prozac. |
| Storage: | | For long term storage -20 degrees Celsius is recommended. Stable at -20 degrees Celsius for at least 1 year. |
| Host_Name: | | Rabbit |
| Buffer: | | 10 mM HEPES [pH 7.5], 150 mM sodium chloride, 100 ug BSA per ml, 50% glycerol |
| ListPrice: | | 295 |
| NovusRef: | | 1. Han, J., et al. Spatial targeting of type II protein kinase A to filopodia mediates the regulation of growth cone guidance by cAMP. J. Cell Biol. 176: 101-111 (2007). |
| AppSummary: | | WB |
| SpeciesSummary: | | Hu, Mu, Rt |
| ALTnames: | | anti-DARPP 32 antibody, anti-DARPP32 antibody, anti-Dopamine- and cAMP-regulated neuronal phosphoprotein antibody |
| ProteinTarget: | | DARPP-32 (Thr34) Antibody |
| PackageSize: | | 0.1 ml |
| Homology: | | 100% homology with bovine, canine, chicken, human, mouse, and non-human primate proteins. |
| Marker: | | Neuronal Marker |
| GeneralRef: | | 1. Svenningsson, P., et al. Diverse psychotomimetics act through a common signaling pathway. Science 302: 1412-1415 (2003). 2. Lindskog, M., et al. Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine. Nature (London) 418: 774-778 (2002). 3. Maldve, R.E., et al. DARPP-32 and the regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens. Nature Neurosci. 5: 641-648 (2002). |
| Phosphorylated: | | Thr34 |
| NotesMain: | | Purification Method: Sequential chromatography on phospho- and non-phosphopeptide affinity columns. Western Blot: Immunolabeling of rat caudate lysates prepared from caudate slices that had been incubated in the absence and presence of forskolin. The labeling by the antibody to DARPP-32 Thr34 is markedly increased by the forskolin treatment. |
| more information: | | company product webpage |