| CatalogCode: | NB300-209 |
| ProductName: | Dynamin Antibody |
| Product Description: | Sheep Polyclonal anti-Dynamin [Ser774] |
| Clonality: | Polyclonal |
| Immunogen: | NB 300-209 was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated Ser774 of rat Dynamin. This is the site phosphorylated by cyclin dependent protein kinase 5 (CDK5). The antigen is identical in human, rat, mouse. |
| Specificity: | Specific for the ~95k dynamin protein phosphorylated at Ser774. Labelsthe purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by lambda-phosphatase treatment. |
| CrossReactivity: | The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse and non-human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen. |
| Packaging: | 0.1 ml Affinity purified Sheep antisera. |
| Uses: | NB 300-209 can be used in Western blot where a band is seen at ~ 97 kD, representing Dynamin.*The investigator should determine the optimal dilution for a specific application. |
| Localization: | Cytoplasmic |
| Background: | Dynamins are a family of GTPases transcribed from at least three separate genes. They contain several conserved regions; an amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a region located in front of the proline-rich domain (PRD). This PRD region is thought to mediate interactions between dynamin and other cellular proteins. There are also at least three isoforms of this protein. Dynamin 2 is ubiquitous. Dynamin 3 is expressed in the brain, testis, heart and lung. Dynamin 1 is exclusively neuronal and is phosphorylated by PKC and dephosphorylated by calcineurin during an action potential in the nerve terminal. It is believed that this dephosphorylation provides a trigger for the cellular process of clatherin-mediated and fluid-phase endocytosis. |
| ProductRef: | 1. Tan, TC, et al. Cdk5 is Essential for Synaptic Vesicle Endocytosis. Nat. Cell Bio., 5(8): 701-710. 2003 |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Affinity purified |
| Host_Name: | Sheep |
| Buffer: | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Rt |
| ALTnames: | Anti-DNM 1 antibody; Anti-DNM antibody; Anti-DNM1 antibody; Anti-Dynamin 1 antibody; Anti-Dynamin1 antibody; Anti-Neuronal dynamin antibody |
| ProteinTarget: | Phospho Dynamin [ Ser 774] antibody |
| PackageSize: | 0.1 ml |
| Phosphorylated: | Ser774 |
| NotesMain: | Prepared from sheep serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. |
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