| Catalog Code: | NB300-208 |
| Product Type: | Primary Antibody |
| Product Name: | Calcium/Calmoduiln kinase II Antibody |
| List Price: | 275 |
| Clonality: | Polyclonal |
| Gene ID: | 5062 |
| Host: | Rabbit |
| Product Description: | Rabbit Polyclonal anti-Calcium/Calmoduiln kinase II [Thr305] |
| Phosphorylated: | Thr305 |
| Cross Reactivity: | NB 300-208 can be detected in rat, mouse, human, bovine, chicken and xenopus. NB 300-208 should not cross-react, as immunolabeling is absent in homogenate of brain from T305D mutant mice. |
| Species Summary: | Rt, Mu, Hu, Bv, Ch, Xp |
| Background: | CaM KII is an important member of calcium/calmodulin-activated kinase, functioning in neural synaptic stimulation and T-cell receptor signaling. CaM KII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releas |
| Immunogen: | NB 300-208 was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated Thr305 of CAM KII. This sequence is identical in human , rat, mouse, drosophila, and limulus and in alpha, beta, and delta forms. |
| Specificity: | NB 300-208 is specific for CAM KII phosphorylated at Thr305. The labeling of the CAM KII bands in Western Blots of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide. |
| Packaging: | 0.1 ml Rabbit antisera. |
| Package Size: | 0.1 ml |
| Uses: | NB 300-208 can be used in Western blot where a band is seen at ~ 50 kD and 60 kD, representing CAM KII. It may also be used in Immunohistochemistry, Immunoprecipitation, and ELISA analysis. Immunofluorescence has not been determined. Suggested working d |
| Application Summary: | WB, IHC, IP, ELISA |
| Product References: | 1. Elgersma, et al. Neuron, 36: 493-505. 2002 |
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