| CatalogCode: | NB300-1065 |
| ProductName: | Estrogen Receptor alpha Antibody |
| Product Description: | Rabbit Polyclonal anti-Estrogen Receptor alpha |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: GGRERLASTNDK, corresponding to amino acids 160-171 of Human Estrogen Receptor (alpha). |
| CrossReactivity: | Cross-reacts with Human. Expected to cross-react with Dog (91% identity with immunogen), Goat (91% identity with immunogen), Pig (91% identity with immunogen), Sheep (91% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | This antibody can be used for ELISA, IP, and Western Blot. Not tested in other applications.Recommended Starting Dilution:*ELISA: Use at a concentration of 0.1 - 1.0 ug/ml. IP: Use 3-5ug for 10<sup>7</sup> cells.WB: Use at a concentration of 0.5 - 2.0 ug/ml. Predicted molecular weight: 67 kDa. *Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Control: | MCF-7 whole cell lysate. |
| Background: | Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS. pH 7.4 |
| ListPrice: | 295 |
| AppSummary: | ELISA, IP, WB |
| SpeciesSummary: | Ca, Hu, Po, Gt, Sh |
| ALTnames: | anti-DKFZp686N23123 antibody, anti-ER alpha antibody, anti-ER antibody, anti-Era antibody, anti-ESR 1 antibody, anti-ESR antibody, anti-ESR1 antibody, anti-ESRA antibody, anti-Estradiol receptor antibody, anti-Estrogen receptor 1 antibody, anti-Estrogen receptor antibody, anti-Major ORF antibody, anti-NR3A1 antibody, anti- antibody |
| ProteinTarget: | Estrogen Receptor alpha |
| PackageSize: | 0.05 mg |
| GeneralRef: | Blobel GA & Orkin SH Estrogen-induced apoptosis by inhibition of the erythroid transcription factor GATA-1. Mol Cell Biol 16:1687-94 (1996). Kraus WL et al. Ligand-dependent, transcriptionally productive association of the amino- and carboxyl-terminal regions of a steroid hormone nuclear receptor. Proc Natl Acad Sci U S A 92:12314-8 (1995). Gaub MP et al. Activation of the ovalbumin gene by the estrogen receptor involves the fos-jun complex. Cell 63:1267-76 (1990). |
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