| CatalogCode: | NB300-1010 |
| ProductName: | Apaf 1 Antibody |
| Product Description: | Rabbit Polyclonal anti-Apaf 1 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide, corresponding to amino acids 1158-1177 of Human Apaf 1. The sequence of the immunogenic peptide differs from that of murine Apaf1 byone amino acid |
| Epitope: | TFYTNGTNLKKIHVSPDFKT |
| CrossReactivity: | Cross-reacts with Human, Rat and Mouse. Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | This antibody can be used for Western Blot. Not tested in other applications.Recommended Starting Dilution:Western blot (1:500 to 1:2000) gives a band at 130 kD.*Optimal dilutions should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homolog of the key cell death gene CED 4 in C. elegans was identified recently from human and mouse and designated APAF1 for Apoptosis protease activating factor 1. APAF1 binds to Cytochrome c (APAF2) and Caspase 9 (APAF3), which leads to Caspase 9 activation. Activated Caspase 9 in turn cleaves and activates Caspase 3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. APAF1 can also associate with Caspase 4 and Caspase 8. APAF1 transcript is ubiquitously expressed in human tissues. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS with 0.02% sodium azide |
| ListPrice: | 295 |
| NovusRef: | 1. Broccardo, Maria. et al. Expression of NK-1 and NK-3 tachykinin receptors in pancreatic acinar cells after acute experimental pancreatitis in rats. 291: G518-G524 (Sep 2006). |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-Apoptotic protease activating factor 1 antibody, anti-CED4 antibody, anti-KIAA0413 antibody |
| ProteinTarget: | Apaf 1 |
| PackageSize: | 0.05 mg |
| GeneralRef: | General / background references:Cecconi F et al. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 94:727-37 (1998). Hu Y et al. Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation. Proc Natl Acad Sci U S A 95:4386-91 (1998). Zou H et al. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 90:405-13 (1997). Li P et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 91:479-89 (1997). |
| NotesMain: | Apoptosis Protease Activating Factor |