| CatalogCode: | NB200-628 |
| ProductName: | PUMA Antibody |
| Product Description: | Rabbit Polyclonal anti-PUMA |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide (PLPRGHRAPEMEPN) corresponding to amino acids 180 to 193 of human PUMA-a (1). This sequence is identical between alpha and beta forms of PUMA proteins. |
| Specificity: | Detects a band at approximately 23 kDa. |
| CrossReactivity: | Cross-reacts with Human and Mouse. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | Western blot: Human K562 or Mouse 3T3 cell lysate can be used as a positive control and a band at approximately 23 kDa can be detected. A lower band at approximately 16 kDa was detected in MOLT4 human and U937 myeloid leukemia cells, which may represent the PUMA-beta form.IHC: Use K562 cells |
| Localization: | Mitochondrial |
| Control: | human K562 or mouse 3T3 |
| Background: | Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS |
| ListPrice: | 325 |
| AppSummary: | IHC, WB |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-p53 Upregulated Modulator of Apoptosis antibody; anti-Bcl-2 binding component 3 antibody; anti-JFY-1 antibody |
| ProteinTarget: | Puma |
| PackageSize: | 0.1 mg |
| GeneralRef: | Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001;7(3):683-94. Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001;7(3):673-82. Han J, Flemington C, Houghton AB , Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001;98(20):11318-23. |
order or
more information: | company product webpage |
| request information: |
|