| Catalog Code: | NB200-621 |
| Product Type: | Primary Antibody |
| Product Name: | Vimentin Antibody |
| List Price: | 325 |
| Clonality: | Monoclonal |
| Gene ID: | 7431 |
| Host: | Mouse |
| Clone: | V9 |
| Isotype: | IgG1 |
| Product Description: | Mouse Monoclonal anti-Vimentin (V9) |
| Cross Reactivity: | Cross-reacts with Human, Rat, Cat, Chicken, Dog, Horse and Pig. Does not cross-react with Mouse or Rat. Not yet tested in other species. |
| Species Summary: | Ca, Ch, Hu, Mu(-), Po, Rt(-), Eq, Fe |
| Background: | Vimentin is the major subunit protein of the intermediate filaments of mesenchumal cells, and has a molecular weight of 57kD. Immunohistochemical staining for Vimentin is thus characteristic of sarcomas (of neural, muscle and fibroblast origin) compared |
| Alternate Names: | anti-FLJ36605 antibody, anti-VIM antibody |
| Localization: | Cytoplasmic |
| Specificity: | The antibody stains the 57 kD vimentin band in immunoblots performed on a lysate of normal human lymphocytes. Does not react with GFAP, neurofilamen, keratin or desmin. |
| Purity: | protein A purified |
| Buffer: | 1% BSA |
| Packaging: | 1.0 ml protein A purified Mouse ascites. |
| Package Size: | 1.0 ml |
| Concentration: | 0.03 mg/ml |
| Uses: | The antibody stains all cells containing vimentin. It can be used on frozen sections and also on formalin fixed and paraffin embedded tissues if pre-treated with trypsin. For use in staining of paraffin sections dilute approximately 1:50 - 1:100, the work |
| Application Summary: | IHC-Fr, IHC-P, WB |
| General References: | General / background references:Osborn M et al. Monoclonal antibodies specific for vimentin. Eur J Cell Biol 34:137-43 (1984). |