| Catalog Code: | NB200-598 |
| Product Type: | Primary Antibody |
| Product Name: | RNA pol II CTD Antibody |
| List Price: | 275 |
| Clonality: | Monoclonal |
| Gene ID: | 5430 |
| Host: | Mouse |
| Clone: | 4H8 |
| Isotype: | IgG1 |
| Product Description: | Mouse Monoclonal anti-RNA pol II CTD (4H8) |
| Cross Reactivity: | Cross-reacts with Human, S. cerevisiae. Expected to cross-react with Drosophilia melanogaster, Hamster, Mouse, S. pombe, Arabidopsis thaliana due to sequence homology - all of these species contain at least one or more 100% identical repeats. Not yet test |
| Species Summary: | Hu, Ye |
| Background: | RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSP |
| Immunogen: | 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-ser 5 YSPTSpPS (Human). |
| Specificity: | This antibody recognises the C-terminal repeat of the largest subunit of RNA polymerase II, it recognises both unphosphorylated and phosphorylated forms. |
| Purity: | Protein A purified |
| Buffer: | Preservative: NoneConstituents: PBS |
| Packaging: | 100 micrograms |
| Package Size: | 0.1 mg |
| Uses: | ChIP: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 217 kDa. Not tested in |
| Application Summary: | ChIP, ELISA, IF, IP, WB |
| General References: | This product has been used in:Kristjuhan A et al. Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation. Proc Natl Acad Sci U S A 100:7551-6 (2003). Kristjuhan A et al. Transcriptional inhibition of genes with severe h |