| CatalogCode: | NB200-559 |
| ProductName: | PRAS40 Antibody |
| Product Description: | Rabbit Polyclonal anti-PRAS40 |
| Clonality: | Polyclonal |
| Immunogen: | This antibody was produced against a chemically synthesized phosphopeptide derived from the region of PRAS40 that contains threonine 246 conjugated to Alexa Fluor |
| CrossReactivity: | Cross-reacts with Human and Mouse. Not yet tested in other species. |
| Packaging: | 0.1 ml Rabbit antisera. |
| Uses: | Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear. Central region of the nuclear pore, within the transporter. During mitoticcell division, it associates with the poles of the mitotic spindle. |
| Control: | NIH3T3 cells. |
| Background: | Proline-Rich Akt Substrate (PRAS40) is a 40 kDa substrate of Akt. Akt and PRAS40 can be found in the phosphoinositide 3-kinase (PI3K) pathway, which plays a role in glucose uptake, cell growth, and apoptosis inhibition. PRAS40 is a 14-3-3 binding protein that reacts with insulin, but whose precise function is not yet known. It has been hypothesized that it may play a role in interacting with SH3 and WW domain containing proteins. The binding of PRAS40 to these proteins may bring about a change of function. Activated Akt phosphorylates PRAS40 on threonine 246, which is thereby able to bind 14-3-3. Mutation of PRAS40 threonine 246 has been shown to be apoptotic, and plays a role in abrogating neuronal protection. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Constituents: 0.2% BSA (without Mg2+ and Ca2+), PBS. pH 7.3 |
| ListPrice: | 310 |
| AppSummary: | IF |
| SpeciesSummary: | Hu, Mu |
| ProteinTarget: | PRAS40 |
| PackageSize: | 0.1 ml |
| GeneralRef: | Kovacina, K.S., et al. (2003) Identification of a proline-rich Akt substrate as a 14-3-3 binding partner. J. Biol. Chem. 278(12):10189-10194. Cantley, L.C. (2002) The phosphoinositide 3-kinase pathway. Science. 296(5573):1655-1657. Lizcano, J.M. and D.R. Alessi (2002) The insulin signaling pathway. Curr. Biol. 12(7):R236-R238. Cho, H., et al. (2001) Akt1/PKBalpha is required for normal growth but dispensable for maintenance of glucose homeostasis in mice. J. Biol. Chem. 276(42):38349-38352. |