| CatalogCode: | NB200-534 |
| ProductName: | GM-CSF Antibody |
| Product Description: | Rat Monoclonal anti-GM-CSF (MP1-31G6) |
| Clone: | MP1-31G6 |
| Clonality: | Monoclonal |
| Immunogen: | This antibody was generated against the recombinant mouse GM-CSF expressed in yeast. It has not been reported to cross-react with rat GM-CSF. |
| CrossReactivity: | Cross-reacts with Mouse. Not yet tested in other species. |
| Packaging: | 0.1 mg IgG purified Rat ascites. |
| Uses: | This antibody can be used in ELISa, Western Blot and Immunoprecipitation.Recommended Starting Dilutions*:WB: 1-5 ug/ml *Optimal dilutions should be determined by the end user. |
| Localization: | Secreted |
| Background: | The purified form of clone MP1-31G6 can be used for: ELISA (capture) in conjunction with antibody from clone MP1-22E9 (1,3-5), ELISPOT, Western blotting. 1-5 ug/ml of antibody can detect less than 100 ng of recombinant mouse GM-CSF under reducing conditions). IMG-227 can also be used for immunohistochemistry of GM-CSF expressing mouse cells, and for immunoprecipitation (4). |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG2a |
| Host_Name: | Rat |
| Buffer: | PBS containing 0.02% sodium azide. Sodium azide is highly toxic. |
| ListPrice: | 295 |
| AppSummary: | ELISA, IP, WB |
| SpeciesSummary: | Mu |
| ALTnames: | anti-Burst Promoting Activity antibody, anti-CMCSF antibody, anti-Colony Stimulating Factor 2 antibody, anti-CSF Alpha antibody, anti-Eosinophil Colony Stimulating Factor antibody, anti-Granulocyte Macrophage Colony Stimulating Factor antibody, anti-Molgramostin antibody, anti-Pluripoietin Alpha antibody, anti-Sargramostim antibody |
| ProteinTarget: | GM-CSF |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1. Sander B, et al. J.Immunol. Meth. 166 : 201-214 (1993). 2. Suda T, et al. Cell. Immunol. 129: 228-240 (1990). 3. Nozaki S, et al. J. Invest. Dermatol. 97: 10-14 (1991). 4. Abrams JS. et al. Immunol. Rev.127:5-24 (1992). 5. Abrams J. In. Current Protocolos in Immunology (J. Cologan et al. Editors) John Wiley and Sons, New York, Unit 6.20 (1995). 6. Litton, et al. J. Immunol. Meth. 175: 47-58 (1994). 7. Guesdon JL, et al. J. Histochem. Cytochem.27: 1131-1139 (1979). |